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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- Ab101688 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- ENO1 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human ENO1 Antibody, Monoclonal (C2), could be used for WB, IHC and so on. Application WB: 0.01-2 µg/mL IHC: 5-20_g/mL Protein Function Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 1-434- Antibody clone number
- C2
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ENO1 Mouse mAb (Ab101688) - Western Blot All lanes: ENO1 Mouse mAb (Ab101688) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 47 kDa Observed band size: 48 kDa Exposure time: 2.5 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ENO1 Mouse mAb (Ab101688) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 20 µg/mL ENO1 Mouse mAb (Ab101688) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- ENO1 Mouse mAb (Ab101688) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 20 µg/mL ENO1 Mouse mAb (Ab101688) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
