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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- RQ4322 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SCRIB Antibody / SCRIBBLE
- Antibody type
- Polyclonal
- Description
- This highly specific SCRIB antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence/Immunocytochemistry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the SCRIB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) MCF7, 2) COLO-320, 3) 22RV1 and 4) SGC-7901 lysate with SCRIB antibody at 0.5ug/ml. Predicted molecular weight ~175 kDa, routinely observed at 200-240 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HepG2 cells with SCRIB antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SCRIB antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A549 cells with SCRIB antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SCRIB antibody.
