PA5-85358

antibody from Invitrogen Antibodies

Targeting: TNNI3 CMD2A, CMH7, TNNC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-85358

Provider

Invitrogen Antibodies

Product name

Cardiac Troponin I Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution. Predicted reactivity: Mouse (93%), Rat (93%), Dog (97%), Cat (97%), Pig (95%), Bovine (97%). Positive Control: 293T, Raji, mouse heart. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.95 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Cardiac Troponin I Polyclonal Antibody (Product # PA5-85358). Sample (30 µg of whole cell lysate). Lane A: Raji. 12% SDS PAGE. Cardiac Troponin I Polyclonal Antibody (Product # PA5-85358) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Cardiac Troponin I Polyclonal Antibody (Product # PA5-85358). Sample (50 µg of whole cell lysate). Lane A: mouse heart. 12% SDS PAGE. Cardiac Troponin I Polyclonal Antibody (Product # PA5-85358) diluted at 1:5,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cardiac Troponin I in A) mouse heart using Cardiac Troponin I polyclonal antibody (Product # PA5-85358) using 50 µg of sample at a dilution of 1:5000. Prior to incubation with primary antibody, the sample was separated on 12% SDS PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cardiac Troponin I in A) Raji cells using Cardiac Troponin I polyclonal antibody (Product # PA5-85358) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 12% SDS PAGE.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Cardiac Troponin I in 4% paraformaldehyde-fixed HeLa cells using Cardiac Troponin I polyclonal antibody (Product # PA5-85358) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cardiac Troponin I Polyclonal Antibody detects Cardiac Troponin I protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cardiac Troponin I protein stained by Cardiac Troponin I Polyclonal Antibody (Product # PA5-85358) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cardiac Troponin I Polyclonal Antibody detects Cardiac Troponin I protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cardiac Troponin I protein stained by Cardiac Troponin I Polyclonal Antibody (Product # PA5-85358) diluted at 1:500. Blue: Hoechst 33342 staining.