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LearnMA5-27676
antibody from Invitrogen Antibodies
Targeting: KCNQ1
JLNS1, KCNA8, KCNA9, Kv7.1, KVLQT1, LQT, LQT1
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- MA5-27676 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KCNQ1 Monoclonal Antibody (N37A/10)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N37A/10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Mechanisms of hearing loss and cell death in the cochlea of connexin mutant mice.
Chen B, Xu H, Mi Y, Jiang W, Guo D, Zhang J, Zhao Y, Tang W
American journal of physiology. Cell physiology 2020 Sep 1;319(3):C569-C578
American journal of physiology. Cell physiology 2020 Sep 1;319(3):C569-C578
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KCNQ1 in hamster T-CHO cell lysate with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with KCNQ1 monoclonal antibody (Product # MA5-27676) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KCNQ1 in human cell lysates with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with KCNQ1 monoclonal antibody (Product # MA5-27676) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KCNQ1 in mouse brain slice. Sample was fixed with 10% formalin (12-24 hours at RT), incubated with KCNQ1 monoclonal antibody (Product # MA5-27676) using a dilution of 1:1000 (1 hour at RT), and followed by Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) and Mayer Hematoxylin (purple/blue) (30 minutes at RT) at a dilution of 250-500 µl.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KCNQ1 in paraffin-embedded human hippocampus. Samples were incubated with KCNQ1 monoclonal antibody (Product # MA5-27676) using a dilution of 1:1000 (1 hour at RT) and followed by FITC Goat Anti-Mouse (green) at a dilution of 1:50 (1 hour at RT).
