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LearnMA5-45407
antibody from Invitrogen Antibodies
Targeting: KCNQ1
JLNS1, KCNA8, KCNA9, Kv7.1, KVLQT1, LQT, LQT1
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- MA5-45407 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KCNQ1 Monoclonal Antibody (N37A/10), APC
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- 1 µg/mL of MA5-45407 was sufficient for detection of KCNQ1 in 10 µg of COS-1 cell lysate transiently expressing KCNQ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 75kDa.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N37A/10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Human Cell lysates showing detection of KCNQ1 protein. Load: 15 µg. Blocking: 1.5% BSA for 30 minutes at RT. Samples were incubated with KCNQ1 monoclonal antibody (Product # MA5-45407) at 1:1,000 for 2 hours at RT, followed by Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with KCNQ1 monoclonal antibody (Product # MA5-45407) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCNQ1 antibody (D) Composite.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using hippocampus. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with KCNQ1 monoclonal antibody (Product # MA5-45407) at 1:1,000 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using Brain Slice. Fixation involved 10% formalin solution for 12-24 hours at RT. Samples were incubated with KCNQ1 monoclonal antibody (Product # MA5-45407) at 1:1,000 for 1 hour at RT, followed by HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain used was Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µL for 5 minutes at RT.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using hippocampus. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with KCNQ1 monoclonal antibody (Product # MA5-45407) at 1:1,000 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
