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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- PA5-37136 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 70 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Proximity Ligation Assay Detection of Protein-DNA Interactions-Is There a Link between Heme Oxygenase-1 and G-quadruplexes?
Krzeptowski W, Chudy P, Sokołowski G, Żukowska M, Kusienicka A, Seretny A, Kalita A, Czmoczek A, Gubała J, Baran S, Klóska D, Jeż M, Stępniewski J, Szade K, Szade A, Grochot-Przęczek A, Józkowicz A, Nowak WN
Antioxidants (Basel, Switzerland) 2021 Jan 12;10(1)
Antioxidants (Basel, Switzerland) 2021 Jan 12;10(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIF1 using PIF1 polyclonal antibody (Product # PA5-37136) at a dilution of 1:500. Lane 1: Hela whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: PC12 whole cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIF1 in Lane 1: HeLa whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: PC12 whole cell lysate. Samples were incubated with PIF1 polyclonal antibody (Product # PA5-37136) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIF1 using PIF1 polyclonal antibody (Product # PA5-37136) at a dilution of 1:500. Lane 1: Hela whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: PC12 whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Expression of Slc48A1 and Slc46A1 in mouse Hmox1 +/+ or Hmox1 -/- HSCs ( A ) and mouse Hmox1 +/+ or Hmox1 -/- iPSCs ( B ). Expression of Pif1 ( C ) and Brip1 ( D ) at the mRNA (graphs) and protein (photos) levels in iPSCs. Gene expression was assessed with RNA-seq in FACS-sorted bone marrow HSCs [] or with real-time RT-PCR in cultured iPSCs. The box-and-whiskers graphs show the median, 25th and 75th percentile (box) minimum, and maximum values (whiskers) of the FPKM (fragments per kilobase million) or relative expression in comparison to house-keeping genes (geometric mean for HPRT, B2M, and beta-actin), n = 4, * p < 0.05, ** p < 0.01, Mann-Whitney test (HSCs) or Wilcoxon test (iPSCs). Representative pictures showing Pif1 and Brip1 immunostaining. Nuclei are labeled with DAPI. Scale bars: 20 um.
