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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [1]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA501694 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501694, RRID:AB_11126225
- Product name
- Anti-SULT2A1 mouse monoclonal antibody, clone OTI4D7 (formerly 4D7)
- Antibody type
- Monoclonal
- Description
- Anti-SULT2A1 mouse monoclonal antibody, clone OTI4D7 (formerly 4D7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SULT2A1
- Isotype
- IgG
- Antibody clone number
- OTI4D7
- Vial size
- 100 µl
- Concentration
- 0.36 mg/ml
Submitted references Upregulation of UGT2B4 Expression by 3'-Phosphoadenosine-5'-Phosphosulfate Synthase Knockdown: Implications for Coordinated Control of Bile Acid Conjugation.
Regulation of murine hepatic hydroxysteroid sulfotransferase expression in hyposulfatemic mice and in a cell model of 3'-phosphoadenosine-5'-phosphosulfate deficiency.
Barrett KG, Fang H, Cukovic D, Dombkowski AA, Kocarek TA, Runge-Morris M
Drug metabolism and disposition: the biological fate of chemicals 2015 Jul;43(7):1061-70
Drug metabolism and disposition: the biological fate of chemicals 2015 Jul;43(7):1061-70
Regulation of murine hepatic hydroxysteroid sulfotransferase expression in hyposulfatemic mice and in a cell model of 3'-phosphoadenosine-5'-phosphosulfate deficiency.
Barrett KG, Fang H, Gargano MD, Markovich D, Kocarek TA, Runge-Morris M
Drug metabolism and disposition: the biological fate of chemicals 2013 Aug;41(8):1505-13
Drug metabolism and disposition: the biological fate of chemicals 2013 Aug;41(8):1505-13
2014-09-04
- Submitted by
- 5408d95cf1031
- Comment
- Diluted SULT2A1 antibody 1:5000, loaded 10 to 20 ug of protein (whole cell lysate) from HepG2 cells. Non-specific binding was not observed. Great success with this antibody.
- Antibody rating
- 5. Excellent
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SULT2A1 (RC204737, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT2A1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SULT2A1 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of SULT2A1 protein level by using anti-SULT2A1 antibody in human HepG2 cells. Dilution: 1:5000
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SULT2A1 mouse monoclonal antibody (TA501694) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SULT2A1(RC204737).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC204737 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SULT2A1 antibody(TA501694), and then analyzed by flow cytometry.
- Validation comment
- FC
