Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA506973 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA506973, RRID:AB_2624018
- Product name
- SERPINB3 mouse monoclonal antibody, clone OTI2H2 (formerly 2H2)
- Antibody type
- Monoclonal
- Description
- SERPINB3 mouse monoclonal antibody, clone OTI2H2 (formerly 2H2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SERPINB3
- Isotype
- IgG
- Antibody clone number
- OTI2H2
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB3 (RC202683, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB3.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Standard curve for ELISA analysis with SCCA (SCCA1+SCCA2) recombinant protein (dilution range from 100pg/ml to 10ug/ml) using SCCA Capture Antibody (Cat# TA506926) at 5ug/ml and HRP conjugated SCCA Detection mAb (Cat# TA506973) at 0.025ug/ml.
- Validation comment
- ELISA
