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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- TA502259 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502259, RRID:AB_11127550
- Product name
- SERPINB3 mouse monoclonal antibody, clone OTI3C2 (formerly 3C2)
- Antibody type
- Monoclonal
- Description
- SERPINB3 mouse monoclonal antibody, clone OTI3C2 (formerly 3C2)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SERPINB3
- Isotype
- IgG
- Antibody clone number
- OTI3C2
- Vial size
- 100 µl
- Concentration
- 0.64 mg/ml
Submitted references A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array.
Zhao Q, Duan R, Yuan J, Quan Y, Yang H, Xi M
International journal of nanomedicine 2014;9:1097-104
International journal of nanomedicine 2014;9:1097-104
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB3 (RC202683, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB3.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SERPINB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502259)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502259)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502259)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: LSPR spectra for processing steps of immobilization and detection of 100 pM SCCa. (A) Bare silver nanochip, ?max =573.92 nm. (B) Modification of 1 mM MUA, ?max =589.1 nm. (C) Incubation with 10 ug/mL monoclonal anti-SCCa, ?max =602.38 nm. (D) Detection of 100 pm SCCa, ?max =612.15 nm.Note: All spectra were collected at room temperature in air.Abbreviations: LSPR, localized surface plasmon resonance; MUA,11-mercaptoundecanoic acid;
- Validation comment
- Assay
