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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00006317-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006317-M01, RRID:AB_548050
- Product name
- Mouse Monoclonal Serpin B3/SCCA1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SERPINB3 - serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01] - Analysis of SERPINB3 expression in transfected 293T cell line by SERPINB3 monoclonal antibody (M01), clone 2F5.Lane 1: SERPINB3 transfected lysate(44.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01] - Detection limit for recombinant GST tagged SERPINB3 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01] - Analysis of SERPINB3 transfected lysate using anti-SERPINB3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SERPINB3 MaxPab rabbit polyclonal antibody.
