17534-1-AP

antibody from Proteintech Group

Targeting: IFT52 C20orf9, CGI-53, dJ1028D15.1, NGD2, NGD5

Provider product page for 17534-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [22]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]

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Product number: 17534-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#17534-1-AP, RRID:AB_2280005
Product name: IFT52 antibody
Antibody type: Polyclonal
Description: IFT52 antibody (Cat. #17534-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, Canine and has been validated for the following applications: IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat, Canine
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

IFT52 protein structure - 17534-1-AP shown in red.

Submitted references PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia.

Zhang T, Cui S, Xiong X, Liu Y, Cao Q, Xia XG, Zhou H
Frontiers in cell and developmental biology 2023;11:1282787

Dynein-2-driven intraciliary retrograde trafficking indirectly requires multiple interactions of IFT54 in the IFT-B complex with the dynein-2 complex.

Hiyamizu S, Qiu H, Tsurumi Y, Hamada Y, Katoh Y, Nakayama K
Biology open 2023 Jul 15;12(7)

Biallelic mutations in CFAP54 cause male infertility with severe MMAF and NOA.

Tian S, Tu C, He X, Meng L, Wang J, Tang S, Gao Y, Liu C, Wu H, Zhou Y, Lv M, Lin G, Jin L, Cao Y, Tang D, Zhang F, Tan YQ
Journal of medical genetics 2023 Aug;60(8):827-834

KLC3 Regulates Ciliary Trafficking and Cyst Progression in CILK1 Deficiency-Related Polycystic Kidney Disease.

Rah G, Cha H, Kim J, Song J, Kim H, Oh YK, Ahn C, Kang M, Kim J, Yoo KH, Kim MJ, Ko HW, Ko JY, Park JH
Journal of the American Society of Nephrology : JASN 2022 Sep;33(9):1726-1741

The intraflagellar transport protein IFT52 associated with short-rib thoracic dysplasia is essential for ciliary function in osteogenic differentiation in vitro and for sensory perception in Drosophila.

Guleria VS, Parit R, Quadri N, Das R, Upadhyai P
Experimental cell research 2022 Sep 15;418(2):113273

Homozygous mutations in CCDC34 cause male infertility with oligoasthenoteratozoospermia in humans and mice.

Cong J, Wang X, Amiri-Yekta A, Wang L, Kherraf ZE, Liu C, Cazin C, Tang S, Hosseini SH, Tian S, Daneshipour A, Wang J, Zhou Y, Zeng Y, Yang S, He X, Li J, Cao Y, Jin L, Ray PF, Zhang F
Journal of medical genetics 2022 Jul;59(7):710-718

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Duan S, Li H, Zhang Y, Yang S, Chen Y, Qiu B, Huang C, Wang J, Li J, Zhu X, Yan X
The EMBO journal 2021 Jan 15;40(2):e105499

Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.

Wang W, Allard BA, Pottorf TS, Wang HH, Vivian JL, Tran PV
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 May;34(5):6369-6381

IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.

Vitre B, Taulet N, Guesdon A, Douanier A, Dosdane A, Cisneros M, Maurin J, Hettinger S, Anguille C, Taschner M, Lorentzen E, Delaval B
EMBO reports 2020 Jun 4;21(6):e49234

Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat.

Zhang Y, Chen Y, Zheng J, Wang J, Duan S, Zhang W, Yan X, Zhu X
Journal of molecular cell biology 2019 May 1;11(5):383-394

Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice.

Liu C, He X, Liu W, Yang S, Wang L, Li W, Wu H, Tang S, Ni X, Wang J, Gao Y, Tian S, Zhang L, Cong J, Zhang Z, Tan Q, Zhang J, Li H, Zhong Y, Lv M, Li J, Jin L, Cao Y, Zhang F
American journal of human genetics 2019 Dec 5;105(6):1168-1181

Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.

Dupont MA, Humbert C, Huber C, Siour Q, Guerrera IC, Jung V, Christensen A, Pouliet A, Garfa-Traoré M, Nitschké P, Injeyan M, Millar K, Chitayat D, Shannon P, Girisha KM, Shukla A, Mechler C, Lorentzen E, Benmerah A, Cormier-Daire V, Jeanpierre C, Saunier S, Delous M
Human molecular genetics 2019 Aug 15;28(16):2720-2737

Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.

Liu W, He X, Yang S, Zouari R, Wang J, Wu H, Kherraf ZE, Liu C, Coutton C, Zhao R, Tang D, Tang S, Lv M, Fang Y, Li W, Li H, Zhao J, Wang X, Zhao S, Zhang J, Arnoult C, Jin L, Zhang Z, Ray PF, Cao Y, Zhang F
American journal of human genetics 2019 Apr 4;104(4):738-748

Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells.

Silva LM, Jacobs DT, Allard BA, Fields TA, Sharma M, Wallace DP, Tran PV
Scientific reports 2018 Mar 21;8(1):4985

FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.

Honda A, Kita T, Seshadri SV, Misaki K, Ahmed Z, Ladbury JE, Richardson GP, Yonemura S, Ladher RK
Proceedings of the National Academy of Sciences of the United States of America 2018 Aug 14;115(33):8388-8393

Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.

Takei R, Katoh Y, Nakayama K
Biology open 2018 Apr 30;7(5)

IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.

Taulet N, Vitre B, Anguille C, Douanier A, Rocancourt M, Taschner M, Lorentzen E, Echard A, Delaval B
Nature communications 2017 Dec 4;8(1):1928

Destabilization of the IFT-B cilia core complex due to mutations in IFT81 causes a Spectrum of Short-Rib Polydactyly Syndrome.

Duran I, Taylor SP, Zhang W, Martin J, Forlenza KN, Spiro RP, Nickerson DA, Bamshad M, Cohn DH, Krakow D
Scientific reports 2016 Sep 26;6:34232

IFT52 mutations destabilize anterograde complex assembly, disrupt ciliogenesis and result in short rib polydactyly syndrome.

Zhang W, Taylor SP, Nevarez L, Lachman RS, Nickerson DA, Bamshad M, University of Washington Center for Mendelian Genomics Consortium, Krakow D, Cohn DH
Human molecular genetics 2016 Sep 15;25(18):4012-4020

Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.

Xu Y, Cao J, Huang S, Feng D, Zhang W, Zhu X, Yan X
PloS one 2015;10(4):e0124378

Active transport and diffusion barriers restrict Joubert Syndrome-associated ARL13B/ARL-13 to an Inv-like ciliary membrane subdomain.

Cevik S, Sanders AA, Van Wijk E, Boldt K, Clarke L, van Reeuwijk J, Hori Y, Horn N, Hetterschijt L, Wdowicz A, Mullins A, Kida K, Kaplan OI, van Beersum SE, Man Wu K, Letteboer SJ, Mans DA, Katada T, Kontani K, Ueffing M, Roepman R, Kremer H, Blacque OE
PLoS genetics 2013;9(12):e1003977

The cilia protein IFT88 is required for spindle orientation in mitosis.

Delaval B, Bright A, Lawson ND, Doxsey S
Nature cell biology 2011 Apr;13(4):461-8

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP(IFT52 antibody) at dilution of 1:400
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using IFT52 antibody 17534-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP result of anti-IFT52 (17534-1-AP for IP and Detection) with HeLa cell lysate.
Sample type: cell line