AP2735c

antibody from Abcepta

Targeting: AKR1B1 ALDR1, AR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2735c

Provider

Abcepta

Proper citation

Abgent Cat#AP2735c, RRID:AB_1208345

Product name

AKR1B1 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of anti-AKR1B1 Pab (Cat.#AP2735c) in Jurkat cell line lysates (35ug/lane).AKR1B1 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of AKR1B1 (arrow) using rabbit polyclonal AKR1B1 Antibody (Center) (Cat.#AP2735c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of AKR1B1 Antibody (Center)(Cat#AP2735c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody (Center) (Cat.#AP2735c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50