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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP12363PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP12363PU-N, RRID:AB_1748141
- Product name
- anti AKR1B1 / ALDR1 (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 287~316 amino acids from the C-terminal region of Human AKR1B1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- 1.89 mg/mL
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-AKR1B1 antibody Cat.-No AP12363PU-N in A375 cell line lysates (35ug/lane). AKR1B1 (arrow) was detected using the purified Pab (1:60 dilution).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using AKR1B1 antibody Cat.-No AP12363PU-N. 293 cell lysates (2ug/lane)either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal Immunofluorescent analysis of AKR1B1 Antibody (C-term) Cat.-No AP12363PU-N with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody (C-term) Cat.-No AP12363PU-N which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
