Explore
Validate
LearnNBP2-17238
antibody from Novus Biologicals
Targeting: MCM2
BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-17238 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal MCM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM2 Antibody [NBP2-17238] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MCM2 Antibody [NBP2-17238] - IP samples: HCT-116 whole cell extract A. 40 ug HCT-116 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of MCM2 protein by 4 ug MCM2 antibody [N1N2], N-term 7.5 % SDS-PAGE The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
