10513-1-AP

antibody from Proteintech Group

Targeting: MCM2 BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030

Provider product page for 10513-1-AP

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Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [56]
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Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 10513-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10513-1-AP, RRID:AB_2142131
Product name: MCM2 antibody
Antibody type: Polyclonal
Description: KD/KO validated MCM2 antibody (Cat. #10513-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MCM2 protein structure - 10513-1-AP shown in red.

Submitted references Claudin18.2 promote gastric cancer proliferation by activating MCM2/5.

Zheng B, Fu M, Lou F, He Y, Zhao L, Huang X, Xie X, Tan W, Chen Q, Zhang W, Hong Y, Rong K, Lu Y, Zhan P, Tu J, Shi H, Hu T, Xiao L
Scientific reports 2026 Feb 23;16(1)

Topoisomerase I Inhibition in ETV4-overexpressed Non-Small Cell Lung Cancer Promotes Replication and Transcription Mediated R-Loop Accumulation and DNA Damage.

Zhang J, Wang Y, Cao S, Xie SM, Liu B, Li Y, Hou Y, Meng X, Ruan M, Bu D, Kang J, Li R, Lou L, Wang J, Xing L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(35):e09307

SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis.

Pan S, Ding F, Hu M, Xue H
Discover oncology 2025 Sep 26;16(1):1732

CRL4(DCAF12) regulation of MCMBP ensures optimal licensing of DNA replication.

Yadav AK, Abdirov A, Ondruskova K, Negi S, Jamrichova K, Kolarova K, Dibus N, Krejci J, Polasek-Sedlackova H, Cermak L
Nature communications 2025 Oct 27;16(1):9391

Small-molecule OPA1 inhibitors reverse mitochondrial adaptations to overcome therapy resistance in acute myeloid leukemia.

La Vecchia S, Doshi S, Antonoglou P, Kundu T, Al Santli W, Avrampou K, Witkowski MT, Pellattiero A, Magrin F, Ames K, Verma A, Gritsman K, Su X, Mattarei A, Aifantis I, Scorrano L, Glytsou C
Science advances 2025 Oct 17;11(42):eadx8662

DNA replication fork speed acts as a pacer in cortical neurogenesis.

Wang J, Kong Y, Li X, Chen D, Xiang K, Tan Y, Shi L
Nature communications 2025 Nov 18;16(1):10121

Large-scale multi-trait genome-wide analysis for inflammatory bowel disease reveals new insights into its molecular mechanisms and emphasizes the roles of systemic immune regulation.

Zhu Z, Shi L, Li H, Wang X, Gao J, Cheng L, Zhang X
Briefings in bioinformatics 2025 Nov 1;26(6)

SETD3-mediated histidine methylation of MCM7 regulates DNA replication by facilitating chromatin loading of MCM.

Duan H, Wang S, Shu WJ, Tong Y, Long HZ, Li G, Du HN, Zhao MJ
Science China. Life sciences 2025 Mar;68(3):793-808

SLFN11-mediated ribosome biogenesis impairment induces TP53-independent apoptosis.

Ogawa A, Izumikawa K, Tate S, Isoyama S, Mori M, Fujiwara K, Watanabe S, Ohga T, Jo U, Taniyama D, Kitajima S, Tanaka S, Onji H, Kageyama SI, Yamamoto G, Saito H, Morita TY, Okada M, Natsumeda M, Nagahama M, Kobayashi J, Ohashi A, Sasanuma H, Higashiyama S, Dan S, Pommier Y, Murai J
Molecular cell 2025 Mar 6;85(5):894-912.e10

TanGIBLE: A selective probe for evaluating hydrophobicity-exposed defective proteins in live cells.

Iwasa Y, Miyata S, Tomita T, Yokota N, Miyauchi M, Mori R, Matsushita S, Suzuki R, Saeki Y, Kawahara H
The Journal of cell biology 2025 Mar 3;224(3)

Significant prognostic value of cell-cycle proteins in early-stage small cell carcinoma of cervix.

Xu Q, Zhou H, Wu J, Chang B, Gu Y, Li H, Cheng X
Scientific reports 2025 Jul 24;15(1):26860

Shikonin inhibits multiple tumor malignant phenotypes and is associated with Hedgehog pathway downregulation in lung adenocarcinoma.

Wang HG, Lu MX, Sheng ML, Lou YB, Xiao YC, Guo A, Yu ZH
Scientific reports 2025 Dec 24;15(1):44516

The EZH2/MCM Complex/hTERT axis facilitates hepatocellular carcinoma progression by inhibiting cellular senescence.

Shen Z, Wang Y, Gao J, Gu W, Ren Z, Xu L, Qian R, Miao Q, Hu X, Wu Y, Liu W, Cai Y, Wan CC, Zhu Y, Sun L, Yan T
Mechanisms of ageing and development 2025 Apr;224:112040

CircPRMT5 promotes progression of osteosarcoma by recruiting CNBP to regulate the translation and stability of CDK6 mRNA.

Liu Y, Jiang H, Hu K, Zou H, Zhang W, Liu J, Jian X
PloS one 2024;19(4):e0298947

RNF8-mediated multi-ubiquitination of MCM7: Linking disassembly of the CMG helicase with DNA damage response in human cells.

Sun Q, Sui Y, Li S, Zhou R, Fu Z, Luo J, Zhao W
Life sciences 2024 Sep 15;353:122912

BET degrader exhibits lower antiproliferative activity than its inhibitor via EGR1 recruiting septins to promote E2F1-3 transcription in triple-negative breast cancer.

Liu N, Wang S, Li M, Zhao N, Wang D, Zhang R, Yu M, Zhao L, Zhang S, Han F, Zhao Y, Liu Q
Pharmacological research 2024 Oct;208:107377

MAT1A activation of glycolysis to promote NSCLC progression depends on stabilizing CCND1.

Shen S, Liu R, Huang J, Sun Y, Tan Q, Luo Q, Liu R
Cell death & disease 2024 Oct 22;15(10):768

YTHDF1 promotes gallbladder cancer progression via post-transcriptional regulation of the m6A/UHRF1 axis.

Chen J, Bai X, Zhang W, Yan Z, Liu Y, Zhou S, Wu X, He X, Yang A
Journal of cellular and molecular medicine 2024 May;28(9):e18328

RNF125‑mediated ubiquitination of MCM6 regulates the proliferation of human liver hepatocellular carcinoma cells.

Feng X, Song D, Liu X, Liang Y, Jiang P, Wu S, Liu F
Oncology letters 2024 Mar;27(3):105

MTCH2 stimulates cellular proliferation and cycles via PI3K/Akt pathway in breast cancer.

Jiang W, Miao Y, Xing X, Liu S, Xing W, Qian F
Heliyon 2024 Mar 30;10(6):e28172

SNRPB2 promotes triple-negative breast cancer progression by controlling alternative splicing of MDM4 pre-mRNA.

Yu S, Si Y, Yu J, Jiang C, Cheng F, Xu M, Fan Z, Liu F, Liu C, Wang Y, Wang N, Liu C, Bi C, Sun H
Cancer science 2024 Dec;115(12):3915-3927

KNTC1 knockdown inhibits the proliferation and migration of osteosarcoma cells by MCM2.

Zhong L, Dong Y, Liu S
Molecular carcinogenesis 2024 Aug;63(8):1599-1610

MFGE8 promotes adult hippocampal neurogenesis in rats following experimental subarachnoid hemorrhage via modifying the integrin β3/Akt signaling pathway.

Li ZY, Yang X, Wang JK, Yan XX, Liu F, Zuo YC
Cell death discovery 2024 Aug 11;10(1):359

ACAT2 suppresses the ubiquitination of YAP1 to enhance the proliferation and metastasis ability of gastric cancer via the upregulation of SETD7.

Zhang M, Cai F, Guo J, Liu S, Ma G, Cai M, Zhang R, Deng J
Cell death & disease 2024 Apr 26;15(4):297

Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2.

Zhang Y, Liu L, Luo B, Tang H, Yu X, Bao S
Oncology research 2023;31(3):317-331

ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway.

Li S, Liu H, Liu W, Shi N, Zhao M, Wanggou S, Luo W, Wang L, Zhu B, Zuo X, Xie W, Zhao C, Zhou Y, Luo L, Gao X, Jiang X, Ren C
International journal of biological sciences 2023;19(3):916-935

circHECTD1 Promotes the Proliferation and Migration of Human Brain Vascular Smooth Muscle Cells via Interacting with KHDRBS3 to Stabilize EZH2 mRNA Expression.

Feng M, Tu W, Zhou Q, Du Y, Xu K, Wang Y
Journal of inflammation research 2023;16:1311-1323

Significance of NotchScore and JAG1 in predicting prognosis and immune response of low-grade glioma.

Shi B, Ge F, Cai L, Yang Y, Guo X, Wu R, Fan Z, Cao B, Wang N, Si Y, Lin X, Dong W, Sun H
Frontiers in immunology 2023;14:1247288

LncRNA AGPG Confers Endocrine Resistance in Breast Cancer by Promoting E2F1 Activity.

Yu S, Wang Y, Gong X, Fan Z, Wang Z, Liang Z, Wu R, Cao B, Wang N, Bi C, Lv D, Sun H
Cancer research 2023 Oct 2;83(19):3220-3236

Molecular subtypes identified by multiomics analysis based on cuproptosis-related genes precisely predict response to immunotherapy and chemotherapy in colorectal cancer.

Li D, Gao W, Zhao W, Zhao Y, Zhang Y, Liu Y, Li Y, Ji S, Chen P, Li D
Molecular carcinogenesis 2023 Nov;62(11):1755-1769

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation.

Xu X, Chang CW, Li M, Omabe K, Le N, Chen YH, Liang F, Liu Y
Nature communications 2023 Mar 4;14(1):1233

Identifying CDC7 as a synergistic target of chemotherapy in resistant small-cell lung cancer via CRISPR/Cas9 screening.

Deng L, Yang L, Zhu S, Li M, Wang Y, Cao X, Wang Q, Guo L
Cell death discovery 2023 Feb 2;9(1):40

O-GlcNAcylation of YTHDF2 promotes HBV-related hepatocellular carcinoma progression in an N(6)-methyladenosine-dependent manner.

Yang Y, Yan Y, Yin J, Tang N, Wang K, Huang L, Hu J, Feng Z, Gao Q, Huang A
Signal transduction and targeted therapy 2023 Feb 10;8(1):63

Hypoxia Inhibits Cell Cycle Progression and Cell Proliferation in Brain Microvascular Endothelial Cells via the miR-212-3p/MCM2 Axis.

Shi Q, Li S, Lyu Q, Zhang S, Bai Y, Ma J
International journal of molecular sciences 2023 Feb 1;24(3)

FANCD2-dependent mitotic DNA synthesis relies on PCNA K164 ubiquitination.

Leung W, Baxley RM, Traband E, Chang YC, Rogers CB, Wang L, Durrett W, Bromley KS, Fiedorowicz L, Thakar T, Tella A, Sobeck A, Hendrickson EA, Moldovan GL, Shima N, Bielinsky AK
Cell reports 2023 Dec 26;42(12):113523

Overlaid Transcriptional and Proteome Analyses Identify Mitotic Kinesins as Important Targets of Arylsulfonamide-Mediated RBM39 Degradation.

Coomar S, Mota P, Penson A, Schwaller J, Abdel-Wahab O, Gillingham D
Molecular cancer research : MCR 2023 Aug 1;21(8):768-778

The Novel lncRNA RP9P Promotes Colorectal Cancer Progression by Modulating miR-133a-3p/FOXQ1 Axis.

Jin Z, Liu B, Lin B, Yang R, Wu C, Xue W, Zou X, Qian J
Frontiers in oncology 2022;12:843064

Environmental exposure to microcystin-LR increases the risks of urinary bladder proliferation and carcinogenesis: Evidence from case control, animal, and in vitro studies.

Pan C, Qin H, Jin H, Chen W, Guo H, Han X
Toxicology 2022 Oct;480:153326

MCM2 promotes the stemness and sorafenib resistance of hepatocellular carcinoma cells via hippo signaling.

Zhou X, Luo J, Xie H, Wei Z, Li T, Liu J, Liao X, Zhu G, Peng T
Cell death discovery 2022 Oct 15;8(1):418

Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes.

Polasek-Sedlackova H, Miller TCR, Krejci J, Rask MB, Lukas J
Nature communications 2022 Oct 14;13(1):6090

CDC7-independent G1/S transition revealed by targeted protein degradation.

Suski JM, Ratnayeke N, Braun M, Zhang T, Strmiska V, Michowski W, Can G, Simoneau A, Snioch K, Cup M, Sullivan CM, Wu X, Nowacka J, Branigan TB, Pack LR, DeCaprio JA, Geng Y, Zou L, Gygi SP, Walter JC, Meyer T, Sicinski P
Nature 2022 May;605(7909):357-365

LncRNA HAR1A Suppresses the Development of Non-Small Cell Lung Cancer by Inactivating the STAT3 Pathway.

Ma J, Cao K, Ling X, Zhang P, Zhu J
Cancers 2022 Jun 8;14(12)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Gourdomichali O, Zonke K, Kattan FG, Makridakis M, Kontostathi G, Vlahou A, Doxakis E
Biology 2022 Feb 11;11(2)

Potential Role of SWI/SNF Complex Subunit Actin-Like Protein 6A in Cervical Cancer.

Wang Q, Cao Z, Wei Y, Zhang J, Cheng Z
Frontiers in oncology 2021;11:724832

Persistent DNA damage alters the neuronal transcriptome suggesting cell cycle dysregulation and altered mitochondrial function.

Vazquez-Villasenor I, Garwood CJ, Simpson JE, Heath PR, Mortiboys H, Wharton SB
The European journal of neuroscience 2021 Nov;54(9):6987-7005

The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.

Li Z, Yin H, Shen Y, Ren M, Xu X
Ecotoxicology and environmental safety 2021 Mar 15;211:111945

Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome.

Liu Y, Zhao Q, Xu F, Wang K, Zhao Y, Chen H, He W, Wang W, Zhang J, Zhang J
Oncology letters 2021 Feb;21(2):117

TRIM21-mediated proteasomal degradation of SAMHD1 regulates its antiviral activity.

Li Z, Huan C, Wang H, Liu Y, Liu X, Su X, Yu J, Zhao Z, Yu XF, Zheng B, Zhang W
EMBO reports 2020 Jan 7;21(1):e47528

Spermidine Prevents Heart Injury in Neonatal Rats Exposed to Intrauterine Hypoxia by Inhibiting Oxidative Stress and Mitochondrial Fragmentation.

Chai N, Zhang H, Li L, Yu X, Liu Y, Lin Y, Wang L, Yan J, Nikolaevna SE, Zhao Y
Oxidative medicine and cellular longevity 2019;2019:5406468

Tmub1 negatively regulates liver regeneration via inhibiting STAT3 phosphorylation.

Fu H, Dong R, Zhang Y, Xu J, Liu M, Chen P
Cellular signalling 2019 Mar;55:65-72

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Song H, Guo T, Zhao Z, Wei Y, Luo H, Weng W, Zhang R, Zhong M, Chen C, Su J, Shen W
Biomaterials 2018 Sep;178:23-35

MCMs expression in lung cancer: implication of prognostic significance.

Liu YZ, Wang BS, Jiang YY, Cao J, Hao JJ, Zhang Y, Xu X, Cai Y, Wang MR
Journal of Cancer 2017;8(18):3641-3647

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Ma Y, Fei L, Zhang M, Zhang W, Liu X, Wang C, Luo Y, Zhang H, Han Y
Oncotarget 2017 Dec 1;8(62):104813-104830

A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens.

Liu YZ, Jiang YY, Wang BS, Hao JJ, Shang L, Zhang TT, Cao J, Xu X, Zhan QM, Wang MR
Cancer cytopathology 2014 Nov;122(11):833-41

Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells.

Valle BL, D'Souza T, Becker KG, Wood WH 3rd, Zhang Y, Wersto RP, Morin PJ
PloS one 2013;8(4):e61836

Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors.

Cunha IW, Carvalho KC, Martins WK, Marques SM, Muto NH, Falzoni R, Rocha RM, Aguiar S, Simoes AC, Fahham L, Neves EJ, Soares FA, Reis LF
Translational oncology 2010 Feb;3(1):23-32

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 10513-1-AP(MCM2 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The MCM2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MCM2. This antibody recognizes human,mouse,rat antigen. The MCM2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 10513-1-AP.pdf

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