LS-C286342

antibody from LSBio

Targeting: MCM2 BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C286342

Provider

LSBio

Product name

MCM2 Antibody (phospho-Ser53) LS-C286342

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human Phospho-MCM2 (S53) by Immunohistochemistry. Samples: FFPE serial sections of human breast adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase treated section (right) immunostained for Phospho-MCM2 (S53). Antibody: Affinity purified rabbit anti-Phospho-MCM2 (S53) used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Mouse Phospho-MCM2 (S53) by Immunohistochemistry. Samples: FFPE serial sections of mouse teratoma. Mock phosphatase treated section (left) or calf intestinal phosphatase treated section (right) immunostained for Phospho-MCM2 (S53). Antibody: Affinity purified rabbit anti-Phospho-MCM2 (S53) used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human Phospho-MCM2 (S53) by Immunohistochemistry. Samples: FFPE serial sections of human breast adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase treated section (right) immunostained for Phospho-MCM2 (S53). Antibody: Affinity purified rabbit anti-Phospho-MCM2 (S53) used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Mouse Phospho-MCM2 (S53) by Immunohistochemistry. Samples: FFPE serial sections of mouse teratoma. Mock phosphatase treated section (left) or calf intestinal phosphatase treated section (right) immunostained for Phospho-MCM2 (S53). Antibody: Affinity purified rabbit anti-Phospho-MCM2 (S53) used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human Phospho-MCM2 (S53) by Immunohistochemistry. Samples: FFPE serial sections of human breast adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase treated section (right) immunostained for Phospho-MCM2 (S53). Antibody: Affinity purified rabbit anti-Phospho-MCM2 (S53) used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.