LS-C287116

antibody from LSBio

Targeting: MCM2 BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Recommended by provider)

Antibody Data

Product number

LS-C287116

Provider

LSBio

Product name

Anti-MCM2 Antibody (aa1-50) LS-C287116

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human MCM2 by Western Blot and Immunoprecipitation. Samples: RIPA extract (50 ug for WB; 7 mg for CoIP) from HeLa cells. Antibody: Affinity purified rabbit anti-MCM2 used at 0.2 ug/ml for WB and 20 ug/7mg extract for IP. Detection: Chemiluminescence for WB; Coomassie staining for CoIP. Identity of CoIP proteins determined by mass spectrometry.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Mouse MCM2 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-MCM2 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human MCM2 by Immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:1000 (1 Detection: DAB.