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LearnGTX103071
antibody from GeneTex
Targeting: MCM2
BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX103071 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103071, RRID:AB_10719639
- Product name
- MCM2 antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
MCM2-regulated functional networks in lung cancer by multi-dimensional proteomic approach.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
MCM2-regulated functional networks in lung cancer by multi-dimensional proteomic approach.
Cheung CHY, Hsu CL, Chen KP, Chong ST, Wu CH, Huang HC, Juan HF
Scientific reports 2017 Oct 16;7(1):13302
Scientific reports 2017 Oct 16;7(1):13302
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE GTX103071 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCM2 antibody [N1N2], N-term detects MCM2 protein at nucleus by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MCM2 antibody [N1N2], N-term immunoprecipitates MCM2 protein in IP experiments.IP samples: HCT-116 whole cell extractA. 40 £gg HCT-116 whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of MCM2 protein by 4 £gg MCM2 antibody [N1N2], N-term (GTX103071)7.5 % SDS-PAGEThe immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
