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LearnPAB10264
antibody from Abnova Corporation
Targeting: MCM2
BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10264 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10264, RRID:AB_1676446
- Product name
- MCM2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MCM2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Interaction of chromatin-associated Plk1 and Mcm7.
Interaction of hRad51 and hRad52 with MCM complex: a cross-talk between recombination and replication proteins.
A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division.
Tsvetkov L, Stern DF
The Journal of biological chemistry 2005 Mar 25;280(12):11943-7
The Journal of biological chemistry 2005 Mar 25;280(12):11943-7
Interaction of hRad51 and hRad52 with MCM complex: a cross-talk between recombination and replication proteins.
Shukla A, Navadgi VM, Mallikarjuna K, Rao BJ
Biochemical and biophysical research communications 2005 Apr 22;329(4):1240-5
Biochemical and biophysical research communications 2005 Apr 22;329(4):1240-5
A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division.
Todorov IT, Pepperkok R, Philipova RN, Kearsey SE, Ansorge W, Werner D
Journal of cell science 1994 Jan;107 ( Pt 1):253-65
Journal of cell science 1994 Jan;107 ( Pt 1):253-65
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using MCM2 polyclonal antibody (Cat # PAB10264) shows detection of both phosphorylated and unphosphorylated MCM2 presentin nuclear extracts from elutriated human cells (MO59K/K-562).The MCM2 protein is phosphorylated after initiation of DNA replication, therefore, the proteinis unphosphorylated in early S phase, and gradually becomes phosphorylated throughout S phase.In G2/M, all MCM2 is phosphorylated.Panel A shows western blot results for lysates were prepared from asynchronous cells (Lane 1), cells arrested in early S with aphidicolin (lane2), and cells arrested in mitosis with nocodazole (Lane 3).Panel B shows a schematic diagram of bands representing phosphorylated and unphosphorylated MCM2 present in these preparations.Asynchronous cells contain a doublet of both forms.Aphidicolin treated cells contain only unphosphorylated MCM2 and nocodozole treatment results in only phosphorylated MCM2 detected in the lysate.The phosphorylated band migrates faster than the unphosphorylated form and isseen as the lower band.There is a clear switch from the unphosphorylated form in the center lane, to the phosphorylated form in the third lane, confirming recognition of both forms of MCM2 by this antibody.The primary antibody was diluted 1:400 for this experiment.Personal Communication, Jennifer Seiler,NIH CCR Bethesda MD.
