Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B14927 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ MCM2 Antibody LS-B14927
- Antibody type
- Polyclonal
- Description
- Caprylic acid and ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of anti-human DNA replication licensing factor MCM2 antibody at 2 ug/ml with HeLa whole cell lysate lane 1: Jurkat whole cell lysate lane 2: K562 whole cell lysate lane 3: MCF-7 whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 102 KDa. Observed band size: 102 KDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate All lanes: MCM2 antibody at 4.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 102 kDa Observed band size: 102 kDa