NB100-639

antibody from Novus Biologicals

Targeting: MCM2 BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-639

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-639, RRID:AB_10000542

Product name

Rabbit Polyclonal MCM2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

HERC2 Interacts with Claspin and regulates DNA origin firing and replication fork progression.

Izawa N, Wu W, Sato K, Nishikawa H, Kato A, Boku N, Itoh F, Ohta T
Cancer research 2011 Sep 1;71(17):5621-5

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Tipin-replication protein A interaction mediates Chk1 phosphorylation by ATR in response to genotoxic stress.

Kemp MG, Akan Z, Yilmaz S, Grillo M, Smith-Roe SL, Kang TH, Cordeiro-Stone M, Kaufmann WK, Abraham RT, Sancar A, Unsal-Kaçmaz K
The Journal of biological chemistry 2010 May 28;285(22):16562-71

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Replication initiation complex formation in the absence of nuclear function in Xenopus.

Krasinska L, Fisher D
Nucleic acids research 2009 Apr;37(7):2238-48

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Plx1 is required for chromosomal DNA replication under stressful conditions.

Trenz K, Errico A, Costanzo V
The EMBO journal 2008 Mar 19;27(6):876-85

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The ATR-mediated S phase checkpoint prevents rereplication in mammalian cells when licensing control is disrupted.

Liu E, Lee AY, Chiba T, Olson E, Sun P, Wu X
The Journal of cell biology 2007 Nov 19;179(4):643-57

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Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases.

Cortez D, Glick G, Elledge SJ
Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 6;101(27):10078-83

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells either mock treated (-) or treated (+) with EPE (100 uM, 2h). Antibodies: MCM2 was immunoprecipitated using rabbit anti-MCM2 antibody was used for WB at 1 mcg/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody . Detection: Chemiluminescence with exposure times of 10 seconds (upper and lower panels).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Detection of Human phospho MCM2 (Ser108) by Western Blot. Samples: Whole cell lysate from HeLa cells that were untreated or exposed to 8 Gy IR for 1 h or 50 J/m2 for 2 hours. Antibody: Affinity purified rabbit anti-phospho MCM2 (Ser108) antibody (Cat. No. NB100-639) used at 0.2 mcg/ml. Subsequently, total MCM2 was detected to verify loading. Detection: Chemiluminescence with an exposure time of 5 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Whole cell lysate (15 or 50 ug) from 293T cells or mouse NIH3T3 (M) cells. Cells were either mock treated (-) or treated (+) with EPE (100 uM, 2h). Antibody used at 0.1 mcg/ml.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MCM2 [p Ser108] Antibody [NB100-639] - Human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB