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LearnNB100-2569
antibody from Novus Biologicals
Targeting: MCM2
BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB100-2569 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2569, RRID:AB_10002735
- Product name
- Rabbit Polyclonal MCM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Cyclin-dependent kinases regulate lysosomal degradation of hypoxia-inducible factor 1α to promote cell-cycle progression.
A nontranscriptional role for HIF-1α as a direct inhibitor of DNA replication.
MCM proteins are negative regulators of hypoxia-inducible factor 1.
Hubbi ME, Gilkes DM, Hu H, Kshitiz, Ahmed I, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):E3325-34
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):E3325-34
A nontranscriptional role for HIF-1α as a direct inhibitor of DNA replication.
Hubbi ME, Kshitiz, Gilkes DM, Rey S, Wong CC, Luo W, Kim DH, Dang CV, Levchenko A, Semenza GL
Science signaling 2013 Feb 12;6(262):ra10
Science signaling 2013 Feb 12;6(262):ra10
MCM proteins are negative regulators of hypoxia-inducible factor 1.
Hubbi ME, Luo W, Baek JH, Semenza GL
Molecular cell 2011 Jun 10;42(5):700-12
Molecular cell 2011 Jun 10;42(5):700-12
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM2 [p Ser41] Antibody [NB100-2569] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MCM2 [p Ser41] Antibody [NB100-2569] - Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) used at a dilution of 1:200 (1 ug/ml).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MCM2 [p Ser41] Antibody [NB100-2569] - Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10e6 cells were stained with 0.1 mcg/ml anti-phospho- MCM2 (pS40/41) in a 150 mcl volume. DNA content was simultaneously analyzed via PI stain
