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LearnNB100-288
antibody from Novus Biologicals
Targeting: MCM2
BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB100-288 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-288, RRID:AB_10003165
- Product name
- Rabbit Polyclonal MCM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Cyclin-dependent kinases regulate lysosomal degradation of hypoxia-inducible factor 1α to promote cell-cycle progression.
A nontranscriptional role for HIF-1α as a direct inhibitor of DNA replication.
Adaptive auto-regulation of androgen receptor provides a paradigm shifting rationale for bipolar androgen therapy (BAT) for castrate resistant human prostate cancer.
A five-gene and corresponding protein signature for stage-I lung adenocarcinoma prognosis.
MCM proteins are negative regulators of hypoxia-inducible factor 1.
Crosslinking of DNA repair and replication proteins to DNA in cells treated with 6-thioguanine and UVA.
The minichromosome maintenance proteins 2-7 (MCM2-7) are necessary for RNA polymerase II (Pol II)-mediated transcription.
Localization of MCM2-7, Cdc45, and GINS to the site of DNA unwinding during eukaryotic DNA replication.
Hubbi ME, Gilkes DM, Hu H, Kshitiz, Ahmed I, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):E3325-34
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):E3325-34
A nontranscriptional role for HIF-1α as a direct inhibitor of DNA replication.
Hubbi ME, Kshitiz, Gilkes DM, Rey S, Wong CC, Luo W, Kim DH, Dang CV, Levchenko A, Semenza GL
Science signaling 2013 Feb 12;6(262):ra10
Science signaling 2013 Feb 12;6(262):ra10
Adaptive auto-regulation of androgen receptor provides a paradigm shifting rationale for bipolar androgen therapy (BAT) for castrate resistant human prostate cancer.
Isaacs JT, D'Antonio JM, Chen S, Antony L, Dalrymple SP, Ndikuyeze GH, Luo J, Denmeade SR
The Prostate 2012 Oct 1;72(14):1491-505
The Prostate 2012 Oct 1;72(14):1491-505
A five-gene and corresponding protein signature for stage-I lung adenocarcinoma prognosis.
Kadara H, Behrens C, Yuan P, Solis L, Liu D, Gu X, Minna JD, Lee JJ, Kim E, Hong WK, Wistuba II, Lotan R
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Mar 15;17(6):1490-501
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Mar 15;17(6):1490-501
MCM proteins are negative regulators of hypoxia-inducible factor 1.
Hubbi ME, Luo W, Baek JH, Semenza GL
Molecular cell 2011 Jun 10;42(5):700-12
Molecular cell 2011 Jun 10;42(5):700-12
Crosslinking of DNA repair and replication proteins to DNA in cells treated with 6-thioguanine and UVA.
Gueranger Q, Kia A, Frith D, Karran P
Nucleic acids research 2011 Jul;39(12):5057-66
Nucleic acids research 2011 Jul;39(12):5057-66
The minichromosome maintenance proteins 2-7 (MCM2-7) are necessary for RNA polymerase II (Pol II)-mediated transcription.
Snyder M, Huang XY, Zhang JJ
The Journal of biological chemistry 2009 May 15;284(20):13466-72
The Journal of biological chemistry 2009 May 15;284(20):13466-72
Localization of MCM2-7, Cdc45, and GINS to the site of DNA unwinding during eukaryotic DNA replication.
Pacek M, Tutter AV, Kubota Y, Takisawa H, Walter JC
Molecular cell 2006 Feb 17;21(4):581-7
Molecular cell 2006 Feb 17;21(4):581-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM2 Antibody [NB100-288] - RIPA extract (50 mcg for WB; 7 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-MCM2 used at 0.2 mcg/ml for WB and 20 mcg/7mg extract for IP
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MCM2 Antibody [NB100-288] - Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for IP at 3 ug per reaction. MCM2 was also immunoprecipitated by rabbit anti-Phospho MCM2 (S108) antibody NB100-639. For blotting immunoprecipitated MCM2, NB100-288 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MCM2 Antibody [NB100-288] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
