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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- R35818 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MKRN1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific MKRN1 antibody is suitable for use in Western blot/Immunofluorescence/Immunocytochemistry/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the MKRN1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of HEK293 lysate over expressing human MKRN1-FLAG tested with MKRN1 antibody (0.3ug/ml) in Lane A and tested with anti-FLAG (1/1000) in lane C. Mock-transfected HEK293 probed with MKRN1 antibody (1ug/ml) in Lane B. Observed molecular weight: 50~60kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse fetal (A) and adult (B) brain lysates with MKRN1 antibody at 1ug/ml. Observed molecular weight: 50~60kDa. The ~35kDa band may be the mouse equivalent to human isoform 2.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human testis tissue lysate with MKRN1 antibody. Predicted molecular weight ~53 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human ThP-1 cell lysate with MKRN1 antibody. Predicted molecular weight ~53 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human U-2 OS cells with MKRN1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human ThP-1 cells with MKRN1 antibody (blue) at 10ug/ml and naive goat Ig (black).
