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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A300-990A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MKRN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse MKRN1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MKRN1 antibody (Product # A300-990A; lot A300-990A-2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MKRN1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and 293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-MKRN1 antibody A300-990A used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MKRN1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- MKRN1 (Cat. No. A300-990A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MKRN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MKRN1 antibody (Product # A300-990A; lot A300-990A-2) used for IP at 6 µg per reaction. MKRN1 was also immunoprecipitated by a previous lot of this antibody (lot A300-990A-1). For blotting immunoprecipitated MKRN1, Product # A300-990A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
