Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-55304 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-55304, RRID:AB_838583
- Product name
- Rabbit Polyclonal MKRN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references MKRN1 induces degradation of West Nile virus capsid protein by functioning as an E3 ligase.
Ko A, Lee EW, Yeh JY, Yang MR, Oh W, Moon JS, Song J
Journal of virology 2010 Jan;84(1):426-36
Journal of virology 2010 Jan;84(1):426-36
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKRN1 Antibody [NB100-55304] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at for WB at 0.1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKRN1 Antibody [NB100-55304] - Detection of human and mouse MKRN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MKRN1 antibody NB100-55304 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MKRN1 Antibody [NB100-55304] - Detection of human MKRN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MKRN1 antibody NB100-55304 (lot NB100-55304-2) used for IP at 6 ug per reaction. MKRN1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-55304-1). For blotting immunoprecipitated MKRN1,NB100-55304 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MKRN1 Antibody [NB100-55304] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- MKRN1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MKRN1 Antibody [NB100-55304] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-MKRN1 used at a dilution of 1:100.