PA5-28461

antibody from Invitrogen Antibodies

Targeting: MKRN1 RNF61

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-28461 - Provider product page
Provider: Invitrogen Antibodies
Product name: MKRN1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: A431, H1299, HeLa, HepG2, rat skin. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.53 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MKRN1 protein structure - PA5-28461 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 IH promotes myocardial apoptosis and downregulates MKRN1. (a) HE staining of heart tissue sections (scale bar, 100 mu m); the blue arrow shows myocardial structure being disorganized; the black arrow shows infiltration of inflammatory cells; (b) representative image of myocardial apoptosis detected using TUNEL staining (scale bar, 20 mu m) and quantification of TUNEL-positive cell nuclei ( n = 6); (c) representative immunoblotting analysis and quantification of MKRN1, Bcl-2, Bax, Bak, p21, cleaved caspase-3, caspase-3 protein levels, and Bax/Bcl-2 ratio in the heart ( n = 6) and GAPDH as an internal control; (d) IHC staining for Bcl-2, Bax, Bak, caspase-3, cleaved caspase-3, and p21 proteins in the heart (scale bar, 100 mu m); (e) MKRN1 mRNA expression in the heart was detected using qPCR ( n = 6); (f) IHC staining for MKRN1 expression in the heart (scale bar, 100 mu m). n indicates the independent sample number in each group. ** p < 0.01.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 IH induces myocardial apoptosis and downregulates MKRN1 H9C2 and AC16 cells were subjected to NC or IH stimulation. (a) Representative immunoblotting analysis and quantification of Bcl-2, Bax, Bak, p21, cleaved caspase-3, caspase-3 protein levels, and Bax/Bcl-2 ratio ( n = 3) and GAPDH as an internal control; (b) representative immunoblotting analysis and quantification of MKRN1 protein expression ( n = 3) and GAPDH as an internal control; (c) MKRN1 mRNA expression was detected using qPCR ( n = 3). n indicates the independent sample number in each group. ** p < 0.01.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 The effect of MKRN1 overexpression in cardiomyocytes is reversed by p21 overexpression under IH conditions. (a, b) H9C2 cells were transfected with control siRNA (siControl) or two kinds of MKRN1-siRNAs (siMKRN1-1 and siMKRN1-2) before IH stimulation. Thereafter, the (a) protein and (b) mRNA expression levels of MKRN1 and p21 were detected using WB and qPCR, respectively ( n = 3); ** p < 0.01 versus the IH + blank group; (c, d) H9C2 cells were transfected with the vector, MKRN1-overexpressing lentiviruses, and MKRN1-overexpressing lentiviruses combined with p21-overexpressing lentiviruses before IH stimulation. (c) Representative immunoblotting analysis and quantification of MKRN1, p21, Bcl-2, Bax, Bak, caspase-3, cleaved caspase-3 protein levels, and the Bax/Bcl-2 ratio ( n = 3) and GAPDH as an internal control; * p < 0.05, ** p < 0.01 versus the NC + vector group, # p < 0.05, ## p < 0.01 versus the IH + vector group, & p < 0.05, and && p < 0.01 versus the IH + MKRN1 group; (d) representative fluorescence image of DHE staining and quantification of fluorescence intensity (scale bar, 100 mu m) ( n = 3); * p < 0.05, ** p < 0.01 versus the NC + vector group, # p < 0.05, ## p < 0.01 versus the IH + vector group, and & p < 0.05 versus the IH + MKRN1 group. n indicates the independent sample number in each group.