MA5-56867

antibody from Invitrogen Antibodies

Targeting: RPS26 eS26, S26

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-56867

Provider

Invitrogen Antibodies

Product name

RPS26 Recombinant Rabbit Monoclonal Antibody (HL3013)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

HL3013

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPS26 using RPS26 Recombinant Monoclonal Antibody (Product # MA5-56867). Neuro2A, NIH-3T3, Raw264.7, and C2C12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPS26 antibody diluted at 1:1,000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPS26 using RPS26 Recombinant Monoclonal Antibody (Product # MA5-56867). PC-12 and Rat2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPS26 antibody diluted at 1:1,000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPS26 using RPS26 Recombinant Monoclonal Antibody (Product # MA5-56867). MCF-7 and MDA-MB-231 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPS26 antibody diluted at 1:1,000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of RPS26 using RPS26 Recombinant Monoclonal Antibody (Product # MA5-56867) in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. RPS26 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (Product # MA5-31466) Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of RPS26 stained by RPS26 Recombinant Monoclonal Antibody (Product # MA5-56867). RPS26 antibody detects RPS26 protein on Paraffin-embedded rat pancreas diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of RPS26 stained by RPS26 Recombinant Monoclonal Antibody (Product # MA5-56867). RPS26 antibody detects RPS26 protein on Paraffin-embedded rat pancreas diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.