A00071

antibody from Boster Biological Technology

Targeting: CYP19A1 ARO, ARO1, aromatase, CPV1, CYAR, CYP19, P-450AROM

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A00071

Provider

Boster Biological Technology

Product name

Anti-Aromatase/CYP19A1 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Aromatase/CYP19A1 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C; for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

METTL3 deficiency leads to ovarian insufficiency due to IL-1β overexpression in theca cells.

Cao M, Yuan C, Chen X, He G, Chen T, Zong J, Shen C, Wang N, Zhao Y, Zhang B, Li C, Zhou X
Free radical biology & medicine 2024 Sep;222:72-84

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Ti(3)C(2) nanosheet-induced autophagy derails ovarian functions.

Yang L, He Z, Hu L, Tang H, Geng Y, Tan Q, Zhang Y, Wen Y, Wu W, Gu H, Liu X
Journal of nanobiotechnology 2024 May 12;22(1):242

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Effects of sub-chronic exposure to microcystin-LR on the endocrine system of male rats.

Wang YT, Wu QH, Chen L, Giesy JP, Xu LL, Xu WL, He J, Shi T, Liu YQ, Xiao SM, Wang YK, Chen F, Chen Y, Xu NH, Ge YL, Chu L, Yan YZ, Chen J, Xie P
The Science of the total environment 2024 Jan 1;906:166839

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miR-21, miR-125b, and let-7b contribute to the involution of atretic follicles and corpus lutea in Tibetan sheep ovaries.

Zhang T, Huo S, Wei S, Cui S
Animal science journal = Nihon chikusan Gakkaiho 2022 Jan-Dec;93(1):e13756

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ETA-mediated anti-TNF-α therapy ameliorates the phenotype of PCOS model induced by letrozole.

Lang Q, Yidong X, Xueguang Z, Sixian W, Wenming X, Tao Z
PloS one 2019;14(6):e0217495

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Decreased hypothalamic aromatization in female rats of true precocious puberty.

Tian ZZ, Zhao H, Chen BY
Neuroscience letters 2004 Aug 5;366(1):92-6

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Electroacupuncture enhances extragonadal aromatization in ovariectomized rats.

Zhao H, Tian Z, Cheng L, Chen B
Reproductive biology and endocrinology : RB&E 2004 Apr 27;2:18

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Aromatase using anti-Aromatase antibody (A00071). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aromatase antigen affinity purified polyclonal antibody (Catalog # A00071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aromatase at approximately 58KD. The expected band size for Aromatase is at 58KD.

Additional image