MA5-33132

antibody from Invitrogen Antibodies

Targeting: CYP19A1 ARO, ARO1, aromatase, CPV1, CYAR, CYP19, P-450AROM

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-33132

Provider

Invitrogen Antibodies

Product name

Aromatase Recombinant Rabbit Monoclonal Antibody (1H1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

1H1

Vial size

100 µL

Concentration

1.75 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Aromatase using a Aromatase Monoclonal antibody (Product # MA5-33132) at a concentration of 2.3 µg/mL. Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate, K562 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 58 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Aromatase using a Aromatase Monoclonal antibody (Product # MA5-33132) at a concentration of 2.3 µg/mL. Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate, K562 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 58 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Aromatase in HepG2 cells using a Aromatase monoclonal antibody (Product # MA5-33132) at a dilution of 1:76. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Cells were counter-stained with DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Aromatase in HepG2 cells using a Aromatase monoclonal antibody (Product # MA5-33132) at a dilution of 1:76. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Cells were counter-stained with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Aromatase in paraffin embedded human placenta tissue using a Aromatase monoclonal antibody (Product # MA5-33132) at a dilution of 1:230. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.