- PA1-16532 - Invitrogen Antibodies CYP19A1 antibody

Submitted references Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

Lu Y, Sareddy GR, Wang J, Zhang Q, Tang FL, Pratap UP, Tekmal RR, Vadlamudi RK, Brann DW
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Sep 16;40(38):7355-7374

Astrocyte-Derived Estrogen Regulates Reactive Astrogliosis and is Neuroprotective following Ischemic Brain Injury.

Wang J, Sareddy GR, Lu Y, Pratap UP, Tang F, Greene KM, Meyre PL, Tekmal RR, Vadlamudi RK, Brann DW
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Dec 9;40(50):9751-9771

Alteration in expression of estrogen receptor isoforms alpha and beta, and aromatase in the testis and its relation with changes in nitric oxide during aging in mice.

Banerjee A, Anjum S, Verma R, Krishna A
Steroids 2012 May;77(6):609-20

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot detection of aromatase in human fetal temporal lobe lysate using Product # PA1-16532. ECL exposure, 1-2 minutes.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Aromatase in human fetal temporal lobe lysate. Samples were incubated in Aromatase polyclonal antibody (Product # PA1-16532). ECL exposure, 1-2 minutes.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1. Attenuated astrocyte activation and aromatization in ovariectomized female FBN-ARO-KO mice after GCI. A , IHC analysis for alterations in astrocyte reactivity and aromatase induction in hippocampal CA1 region at 3, 7, and 14 d after GCI. B , Measurement of hippocampal E2 levels with high-sensitivity E2 ELISA kit at 7 d after GCI. Ca , Representative 3D images of hippocampal CA1 astrocytes with cell body volumes distinguished with different colors. Cb , Astrocyte volumes from the indicated groups were measured and quantitatively analyzed. D , Astrocyte activation was confirmed by examining the expression of two typical astrocyte markers, GFAP and vimentin, with Western blot analysis. E , Astrocyte numbers in hippocampal CA1 region were counted with both GFAP and S100beta immunostaining and expressed as cell numbers per 10 5 um 2 . Values are mean +- SEM of determinations from each group. N = 3-5. * p < 0.05 versus FLOX+Sham. $ p < 0.05 versus FLOX + 3 d. # p < 0.05 versus FLOX + 7 d. & p < 0.05 versus FLOX + 14 d. NS, no significant difference.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7. Male FBN-ARO-KO mice exhibit strong neurodegeneration and cognitive impairment 7 d after GCI injury. Aa , Astrocyte activation and aromatization in hippocampal CA1 region were examined by GFAP and aromatase double staining. Ab , GFAP levels in each group were quantified as a parameter of astrocyte reactivity after GCI injury. Total aromatase levels in hippocampal CA1 region were further measured. B , Astrocyte A1 and A2 phenotypes after GCI were examined by IHC analysis with the markers of C3D ( Ba ) and S100A10 ( Bb ), respectively. Ca , BDNF, IGF-1, and GLT-1 production in astrocytes following the altered astrocyte activation was determined by IHC analysis. Cb , Their relative changes of intensities in astrocytes were quantified. Da , Representative double staining for NeuN and F-Jade C to evaluate neuronal degeneration in each group. Db , F-Jade C-positive neurons were counted. E , Spatial reference memory was assessed by Barnes Maze behavioral test. Ea-Ed , Tracking plots in probe trial. Primary escape latency ( Ee ) and quadrant occupancy ( Ef ) of animals from each group in probe trial were recorded. Values are mean +- SEM of determinations from each group. N = 4 for A-C ; N = 8-11 for D . * p < 0.05 versus FLOX+Sham. # p < 0.05 versus FLOX+GCI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 8. Male FBN-ARO-KO mice display attenuated astrocyte activation, compromised astrocyte functions, and worse neuronal damage at 3 and 14 d after GCI. A , B , GFAP and aromatase double staining to examine astrocyte activation and astrocytic aromatase induction at 3 d ( Aa ) and 14 d ( Ba ) after GCI. Relative changes of GFAP intensities at 3d ( Ab ) and 14d ( Bb ) were quantified. C , Astrocyte-derived BDNF ( Ca ), IGF-1 ( Cb ), and GLT-1 ( Cc ) levels at 3 d after GCI were explored by IHC analysis. D , Their relative intensities in KO + 3 d mice versus FLOX + 3 d mice. E , F , BDNF ( Ea ), IGF-1 ( Eb ), and GLT-1 ( Ec ) levels in hippocampal astrocytes at 14 d after GCI were determined with IHC, and expressed as relative changes of KO + 14 d mice compared with FLOX + 14 d mice ( F ). G , H , Neuronal damage in hippocampal CA1 region at 3 d ( G ) and 14 d ( H ) after GCI was assessed by NeuN and F-Jade C double staining. Values are mean +- SEM of determinations from each group. N = 4. # p < 0.05 versus FLOX + 3 d. & p < 0.05 versus FLOX + 14 d.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 9. Evidence that upregulation of neuronal FGF2 signaling mediates decreased astrocyte activation in ovariectomized female FBN-ARO-KO mice after GCI. Aa , Representative image of FGF2 and NeuN double staining in hippocampal CA1 region. Ab , Quantitative analysis of neuronal FGF2 levels in each group ( N = 4 or 5). B , Neuronal FGF2 alterations in hippocampus were confirmed by Western blotting analysis ( N = 3). C , Representative images of FGFR3 and GFAP double staining showing changes of FGFR3 expression in hippocampal CA1 astrocytes ( N = 4 or 5). Da , Schematic illustration of FGFR3 neutralization by bilateral intracerebroventricular injection of FGFR3 blocking antibody in FBN-ARO-KO GCI mice. Db , The purity of isolated astrocytes from the ischemic brains was evaluated by flow cytometry analysis. E , To examine the effects of FGFR3 neutralization on astrocyte reactivity and functional restoration in FBN-ARO-KO mice after GCI, levels of GFAP, p-STAT3, BDNF, and GLT-1 in purified astrocytes were determined by Western blotting ( Ea ), and further quantitatively analyzed ( Eb ) ( N = 3). Fa , IHC analysis for neuronal degeneration by F-Jade C and NeuN double staining in hippocampal CA1 region. Fb , Quantification of F-Jade C levels in CA1 pyramidal neurons, which was presented as relative intensity of each group versus FLOX+GCI ( N = 4 or 5). Values are mean +- SEM of determinations from each group. * p < 0.05 versus FLOX+Sham. # p < 0.05 versus FLOX+GCI. $ p < 0.05 ver

PA1-16532

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