MA5-24343

AMER1 antibody from Invitrogen Antibodies

FAM123B, FLJ39827, RP11-403E24.2, WTX

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Provider product page for MA5-24343

Antibody Data

Product number

MA5-24343

Provider

Invitrogen Antibodies

Product name

Anti-AMER1 Monoclonal Antibody (729419)

Provider product page

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

729419

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of mouse Anti-human WTX Monoclonal Antibody (Product # MA5-24343) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for WTX at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of WTX was detected in immersion fixed BG01V human embryonic stem cells using mouse Anti-human WTX Monoclonal Antibody (Product # MA5-24343) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-mouse IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of WTX was detected in immersion fixed BG01V human embryonic stem cells using mouse Anti-human WTX Monoclonal Antibody (Product # MA5-24343) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-mouse IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of WTX was detected in immersion fixed BG01V human embryonic stem cells using mouse Anti-human WTX Monoclonal Antibody (Product # MA5-24343) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-mouse IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.