Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA502533 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502533, RRID:AB_11125641
- Product name
- GBA3 mouse monoclonal antibody, clone OTI5G5 (formerly 5G5)
- Antibody type
- Monoclonal
- Description
- GBA3 mouse monoclonal antibody, clone OTI5G5 (formerly 5G5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GBA3
- Isotype
- IgG
- Antibody clone number
- OTI5G5
- Vial size
- 100 µl
- Concentration
- 0.9 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA3 (RC211035, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBA3.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-GBA3 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GBA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502533)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC211035 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GBA3 antibody(TA502533), and then analyzed by flow cytometry.
- Validation comment
- FC
