NBP2-00521

antibody from Novus Biologicals

Targeting: GBA3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-00521

Provider

Novus Biologicals

Product name

Mouse Monoclonal Cytosolic beta-Glucosidase/GBA3 Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1.2 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GBA3 Antibody (1F1) [NBP2-00521] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBA3.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GBA3 Antibody (1F1) [NBP2-00521] Analysis of extracts (35ug) from 9 different cell lines by using anti-GBA3 monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GBA3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GBA3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GBA3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GBA3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Human liver tissue using anti-GBA3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Human lymphoma tissue using anti-GBA3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (1F1) [NBP2-00521] - GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Human prostate tissue using anti-GBA3 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GBA3 Antibody (1F1) [NBP2-00521] - Analysis of Jurkat cells, using anti-GBA3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GBA3 Antibody (1F1) [NBP2-00521] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBA3 antibody, and then analyzed by flow cytometry.