Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC1835P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TGF beta 1/2/3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Exosomes with membrane-associated TGF-β1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction.
Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
Yu L, Yang F, Jiang L, Chen Y, Wang K, Xu F, Wei Y, Cao X, Wang J, Cai Z
European journal of immunology 2013 Sep;43(9):2461-72
European journal of immunology 2013 Sep;43(9):2461-72
Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
Notley CA, Brown MA, Wright GP, Ehrenstein MR
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 15;186(8):4967-72
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 15;186(8):4967-72
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of TGF-beta 1, 2, 3 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-TGF-beta 1, 2, 3 PE-conjugated Mono-clonal Antibody (Catalog # IC1835P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.