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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Flow cytometry [1]
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- Product number
- IC240P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TGF-beta 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant human (rh) TGF-beta 3 and recombinant amphibian TGF-beta 5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references The effect of hepatocyte growth factor on secretory functions in human eosinophils.
Mesenchymal stromal cells induce epithelial-to-mesenchymal transition in human colorectal cancer cells through the expression of surface-bound TGF-β.
Changes of peripheral TGF-β1 depend on monocytes-derived macrophages in Huntington disease.
Myeloid dendritic cells (DCs) of mice susceptible to paracoccidioidomycosis suppress T cell responses whereas myeloid and plasmacytoid DCs from resistant mice induce effector and regulatory T cells.
Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli.
Expression of the T cell receptor αβ on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation.
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Yamauchi Y, Ueki S, Konno Y, Ito W, Takeda M, Nakamura Y, Nishikawa J, Moritoki Y, Omokawa A, Saga T, Hirokawa M
Cytokine 2016 Dec;88:45-50
Cytokine 2016 Dec;88:45-50
Mesenchymal stromal cells induce epithelial-to-mesenchymal transition in human colorectal cancer cells through the expression of surface-bound TGF-β.
Mele V, Muraro MG, Calabrese D, Pfaff D, Amatruda N, Amicarella F, Kvinlaug B, Bocelli-Tyndall C, Martin I, Resink TJ, Heberer M, Oertli D, Terracciano L, Spagnoli GC, Iezzi G
International journal of cancer 2014 Jun 1;134(11):2583-94
International journal of cancer 2014 Jun 1;134(11):2583-94
Changes of peripheral TGF-β1 depend on monocytes-derived macrophages in Huntington disease.
Di Pardo A, Alberti S, Maglione V, Amico E, Cortes EP, Elifani F, Battaglia G, Busceti CL, Nicoletti F, Vonsattel JP, Squitieri F
Molecular brain 2013 Dec 13;6:55
Molecular brain 2013 Dec 13;6:55
Myeloid dendritic cells (DCs) of mice susceptible to paracoccidioidomycosis suppress T cell responses whereas myeloid and plasmacytoid DCs from resistant mice induce effector and regulatory T cells.
Pina A, de Araujo EF, Felonato M, Loures FV, Feriotti C, Bernardino S, Barbuto JA, Calich VL
Infection and immunity 2013 Apr;81(4):1064-77
Infection and immunity 2013 Apr;81(4):1064-77
Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli.
Von Bubnoff D, Scheler M, Wilms H, Fimmers R, Bieber T
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):6701-9
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):6701-9
Expression of the T cell receptor αβ on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
PloS one 2011 Jan 11;6(1):e15997
PloS one 2011 Jan 11;6(1):e15997
Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation.
Cedeno-Laurent F, Barthel SR, Opperman MJ, Lee DM, Clark RA, Dimitroff CJ
Journal of immunology (Baltimore, Md. : 1950) 2010 Oct 15;185(8):4659-72
Journal of immunology (Baltimore, Md. : 1950) 2010 Oct 15;185(8):4659-72
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TGF-beta 1 in PC-3 human prostate cancer cell line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TGF-beta 1 PE-conjugated Monoclonal Antibody (Catalog # IC240P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
