Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- GTX29758 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX29758, RRID:AB_380824
- Product name
- TGF beta 1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis using GTX29758 at 1/200 to detect human TGF1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2).Comparison to molecular weight markers (not shown) was used to estimate the indica
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis using GeneTex's Affinity Purified anti-TGF beta 1 antibody to detect human TGF beta 1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using IRDye? 800 labeled Goat-a-Rabbit IgG (H&L) diluted 1:2,500. IRDye? 800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.