Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA1-197 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TGF beta-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- By Western blot, PA1-197 detects both TGF beta-1 monomer and homodimer.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of TGF beta-1 was performed by loading the indicated amounts of recombinant human active or latent TGF beta-1 protein, 5 µL of serum samples from multiple sclerosis (MS) and leukemia patients, and 15 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with StartingBlock (PBS) Blocking Buffer (Product # 37538) for 1 hour at room temperature. TGF beta-1 was detected at ~12 kD (monomer) and ~24 kD (homodimer) using a TGF beta-1 polyclonal antibody (Product # PA1-197) at a dilution of 1:250 in blocking buffer overnight at 4C on a rocking platform, followed by a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:10,000 for at least 1hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).