MAB7666

antibody from R&D Systems

Targeting: TGFB1 CED, DPD1, TGFB, TGFbeta

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7666

Provider

R&D Systems

Product name

Mouse LAP (TGF-beta 1) Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse LAP (TGF-beta 1) in flow cytometry.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

P04202


Isotype

IgG

Antibody clone number

860206

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

AAV serotype 8-mediated liver specific GNMT expression delays progression of hepatocellular carcinoma and prevents carbon tetrachloride-induced liver damage.

Fang CC, Wu CF, Liao YJ, Huang SF, Chen M, Chen YA
Scientific reports 2018 Sep 14;8(1):13802

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Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient.

Beumer J, Artegiani B, Post Y, Reimann F, Gribble F, Nguyen TN, Zeng H, Van den Born M, Van Es JH, Clevers H
Nature cell biology 2018 Aug;20(8):909-916

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Suppressive IL-17A⁺Foxp3⁺ and ex-Th17 IL-17A^negFoxp3⁺ T_reg cells are a source of tumour-associated T_reg cells.

Downs-Canner S, Berkey S, Delgoffe GM, Edwards RP, Curiel T, Odunsi K, Bartlett DL, Obermajer N
Nature communications 2017 Mar 14;8:14649

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Culture supernatant of adipose stem cells can ameliorate allergic airway inflammation via recruitment of CD4⁺CD25⁺Foxp3 T cells.

Yu HS, Park MK, Kang SA, Cho KS, Mun SJ, Roh HJ
Stem cell research & therapy 2017 Jan 23;8(1):8

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Extracellular Adenosine Production by ecto-5'-Nucleotidase (CD73) Enhances Radiation-Induced Lung Fibrosis.

Wirsdörfer F, de Leve S, Cappuccini F, Eldh T, Meyer AV, Gau E, Thompson LF, Chen NY, Karmouty-Quintana H, Fischer U, Kasper M, Klein D, Ritchey JW, Blackburn MR, Westendorf AM, Stuschke M, Jendrossek V
Cancer research 2016 May 15;76(10):3045-56

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RhoA/phosphatidylinositol 3-kinase/protein kinase B/mitogen-activated protein kinase signaling after growth arrest-specific protein 6/mer receptor tyrosine kinase engagement promotes epithelial cell growth and wound repair via upregulation of hepatocyte growth factor in macrophages.

Lee YJ, Park HJ, Woo SY, Park EM, Kang JL
The Journal of pharmacology and experimental therapeutics 2014 Sep;350(3):563-77

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of LAP (TGF-beta 1) in Mouse iTreg cells by Flow Cytometry. Mouse splenocytes treated with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 μg/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 μg/mL Recombinant Mouse IL-2 (Catalog # 402-IL) for 24 hours to induce T regulatory cells (iTregs) were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7666) or (B) Rat IgG_2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).