Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB246-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LAP (TGF-beta 1) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human LAP in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 27235
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Assessment of Wound-Healing Properties of Medicinal Plants: The Case of Phyllanthus muellerianus.
Reduced CD160 Expression Contributes to Impaired NK-cell Function and Poor Clinical Outcomes in Patients with HCC.
Induction of latency-associated peptide (transforming growth factor-β(1)) expression on CD4+ T cells reduces Toll-like receptor 4 ligand-induced tumour necrosis factor-α production in a transforming growth factor-β-dependent manner.
Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression.
Boakye YD, Agyare C, Ayande GP, Titiloye N, Asiamah EA, Danquah KO
Frontiers in pharmacology 2018;9:945
Frontiers in pharmacology 2018;9:945
Reduced CD160 Expression Contributes to Impaired NK-cell Function and Poor Clinical Outcomes in Patients with HCC.
Sun H, Xu J, Huang Q, Huang M, Li K, Qu K, Wen H, Lin R, Zheng M, Wei H, Xiao W, Sun R, Tian Z, Sun C
Cancer research 2018 Dec 1;78(23):6581-6593
Cancer research 2018 Dec 1;78(23):6581-6593
Induction of latency-associated peptide (transforming growth factor-β(1)) expression on CD4+ T cells reduces Toll-like receptor 4 ligand-induced tumour necrosis factor-α production in a transforming growth factor-β-dependent manner.
Boswell S, Sharif S, Alisa A, Pereira SP, Williams R, Behboudi S
Immunology 2011 Jul;133(3):278-87
Immunology 2011 Jul;133(3):278-87
Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
Blood 2005 Jan 15;105(2):750-8
Blood 2005 Jan 15;105(2):750-8
Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression.
Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G
European journal of immunology 2004 Dec;34(12):3485-96
European journal of immunology 2004 Dec;34(12):3485-96
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LAP (TGF-beta 1) in HEK293 Human Cell Line. LAP (TGF-beta 1) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB246) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LAP TGF-beta 1 Inhibition of TGF-beta 1 Activity and Neutralization by Human LAP TGF-beta 1 Antibody. Recombinant Human LAP TGF-beta 1 (Catalog # 246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (1 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human LAP TGF-beta 1 Monoclonal Antibody (Catalog # MAB246). The ND50 is typically 1-5 µg/mL.