Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AB-245-NA - Provider product page
- Provider
- R&D Systems
- Product name
- TGF-beta 5 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects TGF-beta 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with the other TGF-beta variants is observed. Neutralizes the biological activity of recombinant amphibian TGF-beta 5, but not TGF-beta 1, porcine TGF-beta 1.2, TGF-beta 2, or TGF-beta 3.
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P16176
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- TGF-beta 5 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 5 Antibody. Recombinant Amphibian TGF-beta 5 (Catalog # 245-B5) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Amphibian TGF-beta 5 (0.1 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-TGF-beta 5 Polyclonal Antibody (Catalog # AB-245-NA). The ND50 is typically 0.03-0.08 µg/mL.