IC1835N

antibody from R&D Systems

Targeting: TGFB1 CED, DPD1, TGFB, TGFbeta

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC1835N

Provider

R&D Systems

Product name

TGF-beta 1, 2, 3 Alexa Fluor® 700-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.

Host

Mouse

Conjugate

Near infrared dye

Isotype

IgG

Antibody clone number

1D11

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of TGF-beta 1, 2, 3 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-TGF-beta 1, 2, 3 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC1835N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.