Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- FAB8118A - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse LAP (TGF-beta 1) APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse LAP (TGF-beta 1) in flow cytometry.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Red dye
- Antigen sequence
P04202
- Isotype
- IgG
- Antibody clone number
- 860206
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of LAP (TGF-beta 1) in Mouse CD4+ iTreg cells by Flow Cytometry. Mouse splenocytes treated with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) for 24 hours to generate induced T regulatory cells (iTregs) were stained with Rat Anti-Mouse CD25/IL-2 R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB2438P) and either (A) Rat Anti-Mouse LAP (TGF-beta 1) APC-conjugated Monoclonal Antibody (Catalog # FAB8118A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). Cells were gated on CD4+ iTregs. View our protocol for Staining Membrane-associated Proteins.