Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-9829-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-LAP (Latency Associated peptide) Monoclonal Antibody (FNLAP), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The FNLAP monoclonal antibody reacts with human latency associated peptide (LAP, pro-TGF beta 1, LAP/TGF beta 1). Many different cells produce TGF beta and it mediates effects on the proliferation, differentiation and function of many cell types. TGF beta is synthesized as a precursor that contains LAP at the N-terminus and mature TGF beta at the C-terminus. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells. It is believed that this surface-expressed LAP is due to the binding of LAP to GARP (LRRC32), which is a transmembrane protein that is also found at high levels on platelets and activated regulatory T cells. Applications Reported: This FNLAP antibody has been reported for use in flow cytometric analysis. Applications Tested: This FNLAP antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- FNLAP
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells.
NOTCH1 mediates a switch between two distinct secretomes during senescence.
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Phenotypic and functional characteristics of CD4+ CD39+ FOXP3+ and CD4+ CD39+ FOXP3neg T-cell subsets in cancer patients.
Dawson NA, Lamarche C, Hoeppli RE, Bergqvist P, Fung VC, McIver E, Huang Q, Gillies J, Speck M, Orban PC, Bush JW, Mojibian M, Levings MK
JCI insight 2019 Mar 21;4(6)
JCI insight 2019 Mar 21;4(6)
NOTCH1 mediates a switch between two distinct secretomes during senescence.
Hoare M, Ito Y, Kang TW, Weekes MP, Matheson NJ, Patten DA, Shetty S, Parry AJ, Menon S, Salama R, Antrobus R, Tomimatsu K, Howat W, Lehner PJ, Zender L, Narita M
Nature cell biology 2016 Sep;18(9):979-92
Nature cell biology 2016 Sep;18(9):979-92
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
MacDonald KG, Hoeppli RE, Huang Q, Gillies J, Luciani DS, Orban PC, Broady R, Levings MK
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
Phenotypic and functional characteristics of CD4+ CD39+ FOXP3+ and CD4+ CD39+ FOXP3neg T-cell subsets in cancer patients.
Schuler PJ, Schilling B, Harasymczuk M, Hoffmann TK, Johnson J, Lang S, Whiteside TL
European journal of immunology 2012 Jul;42(7):1876-85
European journal of immunology 2012 Jul;42(7):1876-85
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stimulated with anti-CD3, anti-CD28 and recombinant human IL-2 for 1 day, and then stained with Anti-Human CD4 FITC (Product # 11-0048-42), Anti-Human GARP Alexa Fluor® 647 (Product # 51-9882) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human LAP (Latency Associated Peptide) PE (right). CD4+ cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye