12-9829-42

antibody from Invitrogen Antibodies

Targeting: TGFB1 CED, DPD1, TGFB, TGFbeta

Provider product page for 12-9829-42

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [6]
Comments [0]
Validations
Flow cytometry [1]
Other assay [5]

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Product number: 12-9829-42 - Provider product page
Provider: Invitrogen Antibodies
Product name: LAP (Latency Associated peptide) Monoclonal Antibody (FNLAP), PE, eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: The FNLAP monoclonal antibody reacts with human latency associated peptide (LAP, pro-TGF beta 1, LAP/TGF beta 1). Many different cells produce TGF beta and it mediates effects on the proliferation, differentiation and function of many cell types. TGF beta is synthesized as a precursor that contains LAP at the N-terminus and mature TGF beta at the C-terminus. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells. It is believed that this surface-expressed LAP is due to the binding of LAP to GARP (LRRC32), which is a transmembrane protein that is also found at high levels on platelets and activated regulatory T cells. Applications Reported: This FNLAP antibody has been reported for use in flow cytometric analysis. Applications Tested: This FNLAP antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity: Human
Host: Mouse
Conjugate: Yellow dye
Isotype: IgG
Antibody clone number: FNLAP
Vial size: 100 Tests
Concentration: 5 µL/Test
Storage: 4° C, store in dark, DO NOT FREEZE!

TGFB1 protein structure - 12-9829-42 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 NOTCH1 drives a cell-autonomous senescence with a distinct secretory profile. (a and b) ER:HRAS G12V IMR90 cells, stably expressing N1ICD-FLAG or control vector (V), were incubated with or without 4OHT for 6 days and analysed for expression of indicated proteins by immunoblotting (a), SA-beta-gal and BrdU incorporation (b). One way ANOVA with Dunnett's multiple comparison test; bars are means of >=200 cells, n = 4 biologically independent experiments. *** P
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3. Piezo1 regulates IL-12 and TGFbeta1 production by dendritic cells (DCs) to direct T H 1 and T reg cell differentiation. ( A-B ) Intracellular staining of IL-12p40 and TGFbeta1 expression in WT and Piezo1 -/- splenic DCs after 5 hr of treatment with lipopolysaccharide (LPS) (A; 10 ng/ml) or culture on 2 and 50 kPa hydrogels ( B ). A representative figure shown on the left, and the data summarized on the right. ( C ) Intracellular staining of IFNgamma and Foxp3 in T cells cocultured with WT and Piezo1 -/- splenic DCs in the presence of the indicated treatments (IL-12, Peprotech, 10 mug/ml or anti-TGFbeta1, R&D Systems, 20 mug/ml) for 5 days. A representative figure shown on the left, and the data summarized on the right. ( D ) Intracellular staining of IFNgamma (upper panel) and Foxp3 (lower panel) in T cells cocultured with WT and Piezo1 DC splenic DCs conditioned with 50 kPa hydrogel and the indicated treatments for 5 days and data summarized. The data are representative of three independent experiments (mean +- s.d. ; n=3-5). ***p
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6. Piezo1 regulates TGFbeta1 and IL-12 production through the calcium-calcineurin-NFAT axis. ( A ) Measurement of intracellular Ca 2+ concentrations with Fura2 dye in splenic dendritic cells (DCs) from WT or Piezo1 -/- mice treated with lipopolysaccharide (LPS) (10 ng/ml) or cultured on plates containing 50 kPa hydrogels. ( B ) Intracellular Ca 2+ concentrations measured with Fura2 in splenic DCs from WT mice after the indicated treatment (Yoda1, 25 muM, MCE; ruthenium red, 30 muM, Sigma; gadolinium chloride, 10 muM, Sigma). ( C-D ) Intracellular staining of TGFbeta1 ( C ) and IL-12p40 ( D ) in splenic DCs from WT mice after the indicated treatments. ( E ) Intracellular staining of IL-12p40 in splenic DCs from WT mice after the indicated treatments. A representative figure shown on the left, and the data summarized on the right. ( F ) Intracellular staining of NFATc1 in splenic DCs from WT mice after the indicated treatments (CsA, 10 nM). A representative figure shown on the left, and data summarized on the right. ( G-H ) Intracellular staining of IL-12p40 in splenic DCs from WT or Piezo1 -/- mice after the indicated treatments (Yoda1, 25 muM, MCE; 11R-VIVIT, 100 nM, MCE; CsA, 10 nM, Sigma). A representative figure shown on the left, and data summarized on the right. The data are representative of three to four independent experiments (mean +- s.d. ; n=3-4). ***p
Conjugate: Yellow dye