Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [5]
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- Product number
- 17-9829-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LAP (Latency Associated peptide) Monoclonal Antibody (FNLAP), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The FNLAP monoclonal antibody reacts with human latency associated peptide (LAP, pro-TGF beta 1, LAP/TGF beta 1). Many different cells produce TGF beta and it mediates effects on the proliferation, differentiation and function of many cell types. TGF beta is synthesized as a precursor that contains LAP at the N-terminus and mature TGF beta at the C-terminus. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells. It is believed that this surface-expressed LAP is due to the binding of LAP to GARP (LRRC32), which is a transmembrane protein that is also found at high levels on platelets and activated regulatory T cells. Applications Reported: This FNLAP antibody has been reported for use in flow cytometric analysis. Applications Tested: This FNLAP antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- FNLAP
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Dendritic cell Piezo1 directs the differentiation of T(H)1 and T(reg) cells in cancer.
Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells.
Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.
NOTCH1 mediates a switch between two distinct secretomes during senescence.
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Phenotypic and functional characteristics of CD4+ CD39+ FOXP3+ and CD4+ CD39+ FOXP3neg T-cell subsets in cancer patients.
Transforming growth factor-beta: recent advances on its role in immune tolerance.
GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells.
Amino acid requirements for formation of the TGF-beta-latent TGF-beta binding protein complexes.
TGF-beta: from latent to active.
Wang Y, Yang H, Jia A, Wang Y, Yang Q, Dong Y, Hou Y, Cao Y, Dong L, Bi Y, Liu G
eLife 2022 Aug 22;11
eLife 2022 Aug 22;11
Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells.
Dawson NA, Lamarche C, Hoeppli RE, Bergqvist P, Fung VC, McIver E, Huang Q, Gillies J, Speck M, Orban PC, Bush JW, Mojibian M, Levings MK
JCI insight 2019 Mar 21;4(6)
JCI insight 2019 Mar 21;4(6)
Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.
Pathangey LB, McCurry DB, Gendler SJ, Dominguez AL, Gorman JE, Pathangey G, Mihalik LA, Dang Y, Disis ML, Cohen PA
Oncotarget 2017 Feb 14;8(7):10785-10808
Oncotarget 2017 Feb 14;8(7):10785-10808
NOTCH1 mediates a switch between two distinct secretomes during senescence.
Hoare M, Ito Y, Kang TW, Weekes MP, Matheson NJ, Patten DA, Shetty S, Parry AJ, Menon S, Salama R, Antrobus R, Tomimatsu K, Howat W, Lehner PJ, Zender L, Narita M
Nature cell biology 2016 Sep;18(9):979-92
Nature cell biology 2016 Sep;18(9):979-92
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
MacDonald KG, Hoeppli RE, Huang Q, Gillies J, Luciani DS, Orban PC, Broady R, Levings MK
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
Phenotypic and functional characteristics of CD4+ CD39+ FOXP3+ and CD4+ CD39+ FOXP3neg T-cell subsets in cancer patients.
Schuler PJ, Schilling B, Harasymczuk M, Hoffmann TK, Johnson J, Lang S, Whiteside TL
European journal of immunology 2012 Jul;42(7):1876-85
European journal of immunology 2012 Jul;42(7):1876-85
Transforming growth factor-beta: recent advances on its role in immune tolerance.
Mantel PY, Schmidt-Weber CB
Methods in molecular biology (Clifton, N.J.) 2011;677:303-38
Methods in molecular biology (Clifton, N.J.) 2011;677:303-38
GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells.
Tran DQ, Andersson J, Wang R, Ramsey H, Unutmaz D, Shevach EM
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 11;106(32):13445-50
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 11;106(32):13445-50
Amino acid requirements for formation of the TGF-beta-latent TGF-beta binding protein complexes.
Chen Y, Ali T, Todorovic V, O'leary JM, Kristina Downing A, Rifkin DB
Journal of molecular biology 2005 Jan 7;345(1):175-86
Journal of molecular biology 2005 Jan 7;345(1):175-86
TGF-beta: from latent to active.
Khalil N
Microbes and infection 1999 Dec;1(15):1255-63
Microbes and infection 1999 Dec;1(15):1255-63
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stimulated overnight with Human IL-2 Recombinant Protein (Product # 14-8029-81), Anti-Human CD3, and Anti-Human CD28 Functional Grade Purifieds (Product # 16-0037-81 and Product # 16-0289-81). These cells were then stained with Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), followed by surface staining with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human LAP (Latency Associated Peptide) APC (right), then intracellular staining with Anti-Human Foxp3 PE-eFluor® 610 (Product # 61-4776-42) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Viable CD4+ lymphocytes were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 NOTCH1 drives a cell-autonomous senescence with a distinct secretory profile. (a and b) ER:HRAS G12V IMR90 cells, stably expressing N1ICD-FLAG or control vector (V), were incubated with or without 4OHT for 6 days and analysed for expression of indicated proteins by immunoblotting (a), SA-beta-gal and BrdU incorporation (b). One way ANOVA with Dunnett's multiple comparison test; bars are means of >=200 cells, n = 4 biologically independent experiments. *** P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Piezo1 regulates IL-12 and TGFbeta1 production by dendritic cells (DCs) to direct T H 1 and T reg cell differentiation. ( A-B ) Intracellular staining of IL-12p40 and TGFbeta1 expression in WT and Piezo1 -/- splenic DCs after 5 hr of treatment with lipopolysaccharide (LPS) (A; 10 ng/ml) or culture on 2 and 50 kPa hydrogels ( B ). A representative figure shown on the left, and the data summarized on the right. ( C ) Intracellular staining of IFNgamma and Foxp3 in T cells cocultured with WT and Piezo1 -/- splenic DCs in the presence of the indicated treatments (IL-12, Peprotech, 10 mug/ml or anti-TGFbeta1, R&D Systems, 20 mug/ml) for 5 days. A representative figure shown on the left, and the data summarized on the right. ( D ) Intracellular staining of IFNgamma (upper panel) and Foxp3 (lower panel) in T cells cocultured with WT and Piezo1 DC splenic DCs conditioned with 50 kPa hydrogel and the indicated treatments for 5 days and data summarized. The data are representative of three independent experiments (mean +- s.d. ; n=3-5). ***p
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. Piezo1 regulates TGFbeta1 and IL-12 production through the calcium-calcineurin-NFAT axis. ( A ) Measurement of intracellular Ca 2+ concentrations with Fura2 dye in splenic dendritic cells (DCs) from WT or Piezo1 -/- mice treated with lipopolysaccharide (LPS) (10 ng/ml) or cultured on plates containing 50 kPa hydrogels. ( B ) Intracellular Ca 2+ concentrations measured with Fura2 in splenic DCs from WT mice after the indicated treatment (Yoda1, 25 muM, MCE; ruthenium red, 30 muM, Sigma; gadolinium chloride, 10 muM, Sigma). ( C-D ) Intracellular staining of TGFbeta1 ( C ) and IL-12p40 ( D ) in splenic DCs from WT mice after the indicated treatments. ( E ) Intracellular staining of IL-12p40 in splenic DCs from WT mice after the indicated treatments. A representative figure shown on the left, and the data summarized on the right. ( F ) Intracellular staining of NFATc1 in splenic DCs from WT mice after the indicated treatments (CsA, 10 nM). A representative figure shown on the left, and data summarized on the right. ( G-H ) Intracellular staining of IL-12p40 in splenic DCs from WT or Piezo1 -/- mice after the indicated treatments (Yoda1, 25 muM, MCE; 11R-VIVIT, 100 nM, MCE; CsA, 10 nM, Sigma). A representative figure shown on the left, and data summarized on the right. The data are representative of three to four independent experiments (mean +- s.d. ; n=3-4). ***p