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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [25]
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- Blocking/Neutralizing [1]
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- Product number
- AF-101-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Anti-TGF-beta 1/1.2 Antigen Affinity-purified Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Chinese hamster ovary cell line CHO-derived recombinant human TGF‑β1 (R&D Systems, Catalog # 240-B), Ala270-Ser390
- Description
- Antigen Affinity-purified from egg yolks
- Host
- Chicken/Avian
- Antigen sequence
P01137- Isotype
- IgY
- Vial size
- 100 µg
Submitted references The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells.
The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration.
Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment.
IL-21 induces IL-22 production in CD4+ T cells.
Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor β (TGFβ)-, epidermal growth factor (EGF)-, and nuclear factor κB (NFκB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion.
Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-β.
Long-term gene therapy with thrombospondin 2 inhibits TGF-β activation, inflammation and angiogenesis in chronic allograft nephropathy.
The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression.
Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.
Homeostatic role of transforming growth factor-beta in the oral cavity and esophagus of mice and its expression by mast cells in these tissues.
Characterization of in vitro antimurine thymocyte globulin-induced regulatory T cells that inhibit graft-versus-host disease in vivo.
The LTK63 adjuvant improves protection conferred by Ag85B DNA-protein prime-boosting vaccination against Mycobacterium tuberculosis infection by dampening IFN-gamma response.
TGF-beta promotes thyroid epithelial cell hyperplasia and fibrosis in IFN-gamma-deficient NOD.H-2h4 mice.
Induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production.
Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
The cysteine-rich domain protein KCP is a suppressor of transforming growth factor beta/activin signaling in renal epithelia.
Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure.
Tumor metastasis in an orthotopic murine model of head and neck cancer: possible role of TGF-beta 1 secreted by the tumor cells.
Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
Neuronal TGF-beta1 mediates IL-9/mast cell interaction and exacerbates excitotoxicity in newborn mice.
Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
Autoreactive T cells persist in rats protected against experimental autoimmune encephalomyelitis and can be activated through stimulation of innate immunity.
Autoreactive T cells persist in rats protected against experimental autoimmune encephalomyelitis and can be activated through stimulation of innate immunity.
Effect of neutralizing transforming growth factor beta1 on the immune response against Mycobacterium tuberculosis in guinea pigs.
Inhibition of neointimal hyperplasia after balloon injury by cis-element 'decoy' of early growth response gene-1 in hypercholesterolemic rabbits.
Pakuła M, Mikuła-Pietrasik J, Witucka A, Kostka-Jeziorny K, Uruski P, Moszyński R, Naumowicz E, Sajdak S, Tykarski A, Książek K
International journal of molecular sciences 2019 Jan 2;20(1)
International journal of molecular sciences 2019 Jan 2;20(1)
The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration.
Miroshnychenko O, Chang WT, Dragoo JL
The American journal of sports medicine 2017 Mar;45(4):945-953
The American journal of sports medicine 2017 Mar;45(4):945-953
Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment.
Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, Karpen GH, Blakely EA, Bissell MJ, Barcellos-Hoff MH, Snijders AM, Mao JH
Scientific reports 2015 Mar 9;5:8919
Scientific reports 2015 Mar 9;5:8919
IL-21 induces IL-22 production in CD4+ T cells.
Yeste A, Mascanfroni ID, Nadeau M, Burns EJ, Tukpah AM, Santiago A, Wu C, Patel B, Kumar D, Quintana FJ
Nature communications 2014 May 6;5:3753
Nature communications 2014 May 6;5:3753
Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor β (TGFβ)-, epidermal growth factor (EGF)-, and nuclear factor κB (NFκB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion.
Kang I, Yoon DW, Braun KR, Wight TN
The Journal of biological chemistry 2014 May 30;289(22):15393-404
The Journal of biological chemistry 2014 May 30;289(22):15393-404
Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-β.
Wiener Z, Band AM, Kallio P, Högström J, Hyvönen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
Proceedings of the National Academy of Sciences of the United States of America 2014 May 27;111(21):E2229-36
Proceedings of the National Academy of Sciences of the United States of America 2014 May 27;111(21):E2229-36
Long-term gene therapy with thrombospondin 2 inhibits TGF-β activation, inflammation and angiogenesis in chronic allograft nephropathy.
Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C
PloS one 2013;8(12):e83846
PloS one 2013;8(12):e83846
The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression.
Jelicic K, Cimbro R, Nawaz F, Huang da W, Zheng X, Yang J, Lempicki RA, Pascuccio M, Van Ryk D, Schwing C, Hiatt J, Okwara N, Wei D, Roby G, David A, Hwang IY, Kehrl JH, Arthos J, Cicala C, Fauci AS
Nature immunology 2013 Dec;14(12):1256-65
Nature immunology 2013 Dec;14(12):1256-65
Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.
Oida T, Weiner HL
Journal of immunology (Baltimore, Md. : 1950) 2010 Sep 15;185(6):3529-35
Journal of immunology (Baltimore, Md. : 1950) 2010 Sep 15;185(6):3529-35
Homeostatic role of transforming growth factor-beta in the oral cavity and esophagus of mice and its expression by mast cells in these tissues.
Vitsky A, Waire J, Pawliuk R, Bond A, Matthews D, Lacasse E, Hawes ML, Nelson C, Richards S, Piepenhagen PA, Garman RD, Andrews L, Thurberg BL, Lonning S, Ledbetter S, Ruzek MC
The American journal of pathology 2009 Jun;174(6):2137-49
The American journal of pathology 2009 Jun;174(6):2137-49
Characterization of in vitro antimurine thymocyte globulin-induced regulatory T cells that inhibit graft-versus-host disease in vivo.
Ruzek MC, Waire JS, Hopkins D, Lacorcia G, Sullivan J, Roberts BL, Richards SM, Nahill SR, Williams JM, Scaria A, Dzuris J, Shankara S, Garman RD
Blood 2008 Feb 1;111(3):1726-34
Blood 2008 Feb 1;111(3):1726-34
The LTK63 adjuvant improves protection conferred by Ag85B DNA-protein prime-boosting vaccination against Mycobacterium tuberculosis infection by dampening IFN-gamma response.
Palma C, Iona E, Giannoni F, Pardini M, Brunori L, Fattorini L, Del Giudice G, Cassone A
Vaccine 2008 Aug 5;26(33):4237-43
Vaccine 2008 Aug 5;26(33):4237-43
TGF-beta promotes thyroid epithelial cell hyperplasia and fibrosis in IFN-gamma-deficient NOD.H-2h4 mice.
Yu S, Sharp GC, Braley-Mullen H
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 1;181(3):2238-45
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 1;181(3):2238-45
Induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production.
Tabata T, Kawakatsu H, Maidji E, Sakai T, Sakai K, Fang-Hoover J, Aiba M, Sheppard D, Pereira L
The American journal of pathology 2008 Apr;172(4):1127-40
The American journal of pathology 2008 Apr;172(4):1127-40
Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2006 May;31(5):516-22
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2006 May;31(5):516-22
The cysteine-rich domain protein KCP is a suppressor of transforming growth factor beta/activin signaling in renal epithelia.
Lin J, Patel SR, Wang M, Dressler GR
Molecular and cellular biology 2006 Jun;26(12):4577-85
Molecular and cellular biology 2006 Jun;26(12):4577-85
Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure.
Neurohr C, Nishimura SL, Sheppard D
American journal of respiratory cell and molecular biology 2006 Aug;35(2):252-9
American journal of respiratory cell and molecular biology 2006 Aug;35(2):252-9
Tumor metastasis in an orthotopic murine model of head and neck cancer: possible role of TGF-beta 1 secreted by the tumor cells.
Dasgupta S, Bhattacharya-Chatterjee M, O'Malley BW Jr, Chatterjee SK
Journal of cellular biochemistry 2006 Apr 1;97(5):1036-51
Journal of cellular biochemistry 2006 Apr 1;97(5):1036-51
Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
Blood 2005 Jan 15;105(2):750-8
Blood 2005 Jan 15;105(2):750-8
Neuronal TGF-beta1 mediates IL-9/mast cell interaction and exacerbates excitotoxicity in newborn mice.
Mesplès B, Fontaine RH, Lelièvre V, Launay JM, Gressens P
Neurobiology of disease 2005 Feb;18(1):193-205
Neurobiology of disease 2005 Feb;18(1):193-205
Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
Helanterä I, Loginov R, Koskinen P, Törnroth T, Grönhagen-Riska C, Lautenschlager I
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2005 Apr;20(4):790-6
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2005 Apr;20(4):790-6
Autoreactive T cells persist in rats protected against experimental autoimmune encephalomyelitis and can be activated through stimulation of innate immunity.
Conant SB, Swanborg RH
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5322-8
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5322-8
Autoreactive T cells persist in rats protected against experimental autoimmune encephalomyelitis and can be activated through stimulation of innate immunity.
Conant SB, Swanborg RH
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5322-8
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5322-8
Effect of neutralizing transforming growth factor beta1 on the immune response against Mycobacterium tuberculosis in guinea pigs.
Allen SS, Cassone L, Lasco TM, McMurray DN
Infection and immunity 2004 Mar;72(3):1358-63
Infection and immunity 2004 Mar;72(3):1358-63
Inhibition of neointimal hyperplasia after balloon injury by cis-element 'decoy' of early growth response gene-1 in hypercholesterolemic rabbits.
Ohtani K, Egashira K, Usui M, Ishibashi M, Hiasa KI, Zhao Q, Aoki M, Kaneda Y, Morishita R, Takeshita A
Gene therapy 2004 Jan;11(2):126-32
Gene therapy 2004 Jan;11(2):126-32
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- TGF-beta 1 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 1/1.2 Antibody. Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Chicken Anti-TGF-beta 1/1.2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-101-NA). The ND50 is typically 5-30 ng/mL.
