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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [1]
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- Product number
- IC240A - Provider product page
- Provider
- R&D Systems
- Product name
- Human TGF-beta 1 APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant human (rh) TGF-beta 3 and recombinant amphibian TGF-beta 5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- 9016
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references CD8+ T cells with high TGF‑β1 expression cause lymph node fibrosis following HIV infection.
Induction of Immunosuppressive CD8+CD25+FOXP3+ Regulatory T Cells by Suboptimal Stimulation with Staphylococcal Enterotoxin C1.
B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity.
Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation.
Huang L, Deng J, Xu W, Wang H, Shi L, Wu F, Wu D, Nei W, Zhao M, Mao P, Zhou X
Molecular medicine reports 2018 Jul;18(1):77-86
Molecular medicine reports 2018 Jul;18(1):77-86
Induction of Immunosuppressive CD8+CD25+FOXP3+ Regulatory T Cells by Suboptimal Stimulation with Staphylococcal Enterotoxin C1.
Lee J, Park N, Park JY, Kaplan BLF, Pruett SB, Park JW, Park YH, Seo KS
Journal of immunology (Baltimore, Md. : 1950) 2018 Jan 15;200(2):669-680
Journal of immunology (Baltimore, Md. : 1950) 2018 Jan 15;200(2):669-680
B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity.
Piancone F, Saresella M, Marventano I, La Rosa F, Zoppis M, Agostini S, Longhi R, Caputo D, Mendozzi L, Rovaris M, Clerici M
Scientific reports 2016 Jul 14;6:29699
Scientific reports 2016 Jul 14;6:29699
Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation.
Cedeno-Laurent F, Barthel SR, Opperman MJ, Lee DM, Clark RA, Dimitroff CJ
Journal of immunology (Baltimore, Md. : 1950) 2010 Oct 15;185(8):4659-72
Journal of immunology (Baltimore, Md. : 1950) 2010 Oct 15;185(8):4659-72
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of TGF-beta 1 in PC-3 human prostate cancer cell line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TGF-beta 1 APC-conjugated Monoclonal Antibody (Catalog # IC240A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
