PAB24335

antibody from Abnova Corporation

Targeting: NOL8 C9orf34, FLJ20736, Nop132

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB24335

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB24335, RRID:AB_11121378

Product name

NOL8 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NOL8.

Antigen sequence

TDFSLSISNSSDVSAKDKHAEDNEKRLAALEARQK
AKEVQKKLVHNALANLDGHPEDKPTHIIFGSDSEC
ETEETSTQEQSHPGEEWVKESMGKTSGKLFDSSDD
DESDSEDDSNRFKIKPQFEGRAGQKLMDLQSHFGT
DDRFRMDSR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NOL8 polyclonal antibody (Cat # PAB24335) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with NOL8 polyclonal antibody (Cat # PAB24335) at 1-4 ug/mL dilution shows positivity in nucleus and nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with NOL8 polyclonal antibody (Cat # PAB24335) shows strong cytoplasmic and nuclear positivity in purkinje cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)