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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Other assay [2]
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- Product number
- MA1-21190 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Fos Monoclonal Antibody (2G9C3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody does not cross-react with the 39 kDa jun protein. By Western blot, this antibody detects ~55 & 62 kDa bands representing c-fos and v-fos, respectively. MA1-21190 is not suitable for IHC applications on frozen or paraffin tissues. Positive control of HeLa Cells suggested.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G9C3
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Fructose 1,6-Bisphosphatase 2 Plays a Crucial Role in the Induction and Maintenance of Long-Term Potentiation.
Sensory Ganglia-Specific TNF Expression Is Associated With Persistent Nociception After Resolution of Inflammation.
Duda P, Wójtowicz T, Janczara J, Krowarsch D, Czyrek A, Gizak A, Rakus D
Cells 2020 Jun 1;9(6)
Cells 2020 Jun 1;9(6)
Sensory Ganglia-Specific TNF Expression Is Associated With Persistent Nociception After Resolution of Inflammation.
Gonçalves WA, Rezende BM, de Oliveira MPE, Ribeiro LS, Fattori V, da Silva WN, Prazeres PHDM, Queiroz-Junior CM, Santana KTO, Costa WC, Beltrami VA, Costa VV, Birbrair A, Verri WA Jr, Lopes F, Cunha TM, Teixeira MM, Amaral FA, Pinho V
Frontiers in immunology 2019;10:3120
Frontiers in immunology 2019;10:3120
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Expression of the late-phase LTP depends on Fbp2 structure and concentration. ( A ) Induction of LTP increases fluorescence related to c-Fos (left panel) and c-Jun (right panel); however, inhibition of Fbp or reduced concentration of the enzyme abolishes the expression of both the transcription factors. ( B ) Quantification of mean c-Fos- and c-Jun-associated fluorescence in neurons after the LTP induction as compared to the fluorescence in neurons in which LTP was not induced. For each condition, N = 30 ROIs. ( C ) Nuclear translocation of Camk4 is blocked by Fbp inhibition/tetramerization or silencing. ( D ) Mean Camk4-associated fluorescence from neuronal nuclei after the LTP induction as compared with that observed in unstimulated neurons (control). For each condition, N = 20 ROIs. Bar = 20 mum. The values are given as a mean and SD. * indicates p < 0.05, ** indicates p < 0.01.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Persistent articular nociception in AIA model. Immunized mice received either i.a. injection of mBSA (10 mug/10 mul of PBS) to AIA induction or PBS (10 mul) in the control group. (A) Time course of nociceptive responses was recorded by electronic von Frey algesimeter. BL (baseline) ( n = 6). (B) Confocal microscopy slides show c-Fos positive neurons within DRG (L4) 8 days after AIA induction and (C) its quantification. DRG samples from the control group were extracted 8 days after i.a. injection ( n = 5-6). The results are represented as mean +- SEM; **** p < 0.0001 (A) or p = specified value in the graph (C) compared to control group (PBS) using two-way ANOVA following by Sidak post-hoc analysis (A) or by Dunnett post-hoc analysis (C) .
